Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea

Proceedings of a Workshop on Diagnostic Techniques for Enteropathogenic Agents Associated with Neonatal Diarrhoea in Calves and Pigs, held at the Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands, June 3-5, 198
Softcover Nachdruck of the original 1. Auflage 1981
ISBN: 978-94-009-8330-4
Verlag: Springer Netherlands

Buch, Englisch, Band 13, 210 Seiten, Format (B × H): 156 mm x 244 mm, Gewicht: 351 g

Reihe: Current Topics in Veterinary Medicine

In recent years several "new" infectious agenLs have been associated with neonatal diarrhoea in both calves and pigs. Furthermore, important additional information has become available as regards enterotoxigenic Escherichia coli infec­ tions in both species. Although still much has to be learned, it is likely that in many field cases differnt agents act in concert. Therefore an integrated approach to the problem of neonatal diarrhoea appears to be necessary, particularly in the field research. Such an approach requires a series of diagnostic techniques. The purpose of this meeting was to bring together a limited number of scientists that are actively involved in neonatal diarrhoea research, in order to discuss present knowledge and to produce proceedings containing review articles, new developments and laboratory manuals of relevant diagnostic techniques. x This publication constitutes a collection of scientific pa­ pers and laboratory manuals on diagnostic techniques for en­ teropathogenic agents in neonatal diarrhoea in calves and pigs, presented and discussed during a workshop in the EEC Program­ me of Coordination of Agricultural Research on Protection of the Young Animal against Perinatal Diseases, held at the Central Veterinary Institute, Department of Virology, Lely­ stad, the Netherlands, June 3-5, 1980. VIRAL AGENTS ASSOCIA1ED WIlli NEONATAL DIARRHOEA AND TIlEIR DE1ECTION BY ELECTRON MICROSCOPY M. S. McNulty, W. 1. Curran and J. B. McFerran Veterinary Research Laboratories, Stormont, Belfast, BT4 3SD, N. Ireland ABSTRACT Simple techniques for diagnosis of enteric viral infections by direct electron microscopy of faeces are described.

Zielgruppe

Research

Autoren/Hrsg.

Guinee, P. A. M.

de Leeuw, Peter W.

Inhaltsverzeichnis

Enteric Viruses.- Papers presented.- Viral agents associated with neonatal diarrhoea and their detection by electron microscopy.- Cell culture techniques for the identification of enteric viruses — present possibilities.- Diagnosis by enzyme-linked immunosorbent assay.- Application of purified 5-aminosalicylic acid as substrate in enzyme-linked immunosorbent assay (ELISA).- ELISA for the detection of rotavirus and rotavirus/antibody complexes in faeces.- RNA gel electrophoresis: a technique to monitor laboratory cross-contaminations and genetic reassortment amongst rotavirus isolates.- The neutralisation of the indirect immunofluorescence test and the immuno-diffusion test: two detection methods for neonatal calf diarrhoea viruses. A comparative study with ELISA and electron microscopy.- Comparison of diagnostic procedures for bovine rotavirus.- The diagnosis of Coronavirus-like agent (CVLA) diarrhea in suckling pigs.- Comparison of diagnostic techniques for research in neonatal diarrhea.- Laboratory manuals.- Agricultural Research Council’s Institute for Research on Animal Diseases, Compton, Nr. Newbury, Berks., U.K.:.- Station de Recherches de Virologie et d’Immunologie INRA, 78850-Thiverval-Grignon, France:.- Central Veterinary Institute, Virology Department, 8221 RA Lelystad, the Netherlands:.- Central Veterinary Institute, the Netherlands and Institute of Medical Microbiology, University of Copenhagen, Department of Clinical Virology, EK-2100 Copenhagen, Denmark:.- Institute of Medical Microbiology, Copenhagen, Denmark:.- National Institute for Veterinary Research, Groeselenberg 99, 1180 Brussels, Belgium:.- Laboratory for Virology, Faculteit van de Diergeneeskunde, B 9000 Gent, Belgium:.- Enteropathogenic E. Coli.- Escherichia coli associated withneonatal diarrhoea in piglets and calves.- Adhesion of Escherichia coli pathogenic in pigs, calves and lambs to intestinal epithelial cell brush-borders.- Manual of the brush-border test.- Preliminary experiences with an enzyme-linked immunosorbent assay (ELISA) for the detection of the K88 antigen of E. coli in porcine faeces.- Manual of the K88 ELISA.- Hydrophobic interaction chromatography (laboratory manual).- Miscellaneous.- Mixed diarrhoeal infection in calves: the relative importance of 2 interacting enteropathogens.- Piglet enteritis; field and laboratory evaluation of enteropathogens associated with 3 disease entities.- Cryptosporidiosis in calves: significance within the enteritis syndrome and diagnosis of infection.- List of Participants.