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Genomic Imprinting

Methods and Protocols

  • Book
  • © 2002

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Editors:
  1. Andrew Ward

    1. Department of Biology and Biochemistry, University of Bath, Claverton Down, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 181)

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About this book

Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand.

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Generation of Monoparental Embryos for Investigation into Genomic Imprinting

    • Wendy L. Dean, Gavin Kelsey, Wolf Reik
    Pages 1-19

  3. Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting

    • Jeffrey R. Mann
    Pages 21-39

  4. Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome

    • Anne C. Ferguson-Smith, Maxine Tevendale, Pantelis Georgiades, Valerie Grandjean
    Pages 41-54

  5. Production of YAC Transgenic Mice by Pronuclear Injection

    • Justin F.-X. Ainscough, Rosalind M. John, Sheila C. Barton
    Pages 55-65

  6. A Transgenic Approach to Studying Imprinted Genes

    • Rosalind M. John, Justin F.-X. Ainscough, Sheila C. Barton
    Pages 67-81

  7. Methylation-Sensitive Genome Scanning

    • Izuho Hatada, Tsunehiro Mukai
    Pages 83-100

  8. Subtraction-Hybridization Method for the Identification of Imprinted Genes

    • Fumitoshi Ishino, Yoshimi Kuroiwa, Naoki Miyoshi, Shin Kobayashi, Takashi Kohda, Tomoko Kaneko-Ishino
    Pages 101-112

  9. Identification of Imprinted Loci by Methylation

    • Rachel J. Smith, Gavin Kelsey
    Pages 113-132

  10. Ribonuclease Protection

    • Joanne L. Thorvaldsen, Marisa S. Bartolomei
    Pages 133-144

  11. Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression

    • Judith Singer-Sam, Chunguang Gao
    Pages 145-152

  12. Allele-Specific In Situ Hybridization (ASISH)

    • Rolf Ohlsson, Kristian Svensson, Hengmi Cui, Helena Malmikumpu, Gail Adam
    Pages 153-167

  13. RNA-FISH to Analyze Allele-Specific Expression

    • Giovanna Braidotti
    Pages 169-180

  14. Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes

    • Janine LaSalle, Marc Lalande
    Pages 181-192

  15. Southern Analysis Using Methyl-Sensitive Restriction Enzymes

    • Tom Moore
    Pages 193-203

  16. A PCR-Based Method for Studying DNA Methylation

    • Mira Ariel
    Pages 205-216

  17. Bisulfite-Based Methylation Analysis of Imprinted Genes

    • Sabine Engemann, Osman El-Maarri, Petra Hajkova, Joachim Oswald, Joern Walter
    Pages 217-228

  18. Direct Analysis of Chromosome Methylation

    • Déborah Bourc’his, Evani Viegas-Péquignot
    Pages 229-242

  19. In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs

    • Ilse Van den Wyngaert, Roger L. P. Adams, Stefan U. Kass
    Pages 243-250

  20. In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation

    • Ghislaine Dell, Marika Charalambous, Andrew Ward
    Pages 251-258
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Reviews

"...a multi-authored volume in which the methods of gene imprinting are discussed in detail in 25 chapters. The protocols are very detailed with multiple schemes and figures." - Journal of Pediatric Endocrinology and Metabolism

Editors and Affiliations

  • Department of Biology and Biochemistry, University of Bath, Claverton Down, UK

    Andrew Ward

Bibliographic Information

  • Book Title: Genomic Imprinting

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew Ward

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592112

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-741-0Published: 10 October 2001

  • Softcover ISBN: 978-1-61737-164-6Published: 10 November 2010

  • eBook ISBN: 978-1-59259-211-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 377

  • Topics: Human Genetics

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