Skip to main content
Springer Nature Link
Log in

Reverse Genetics of RNA Viruses

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Daniel R. Perez

    1. Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1602)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Similar content being viewed by others

Keywords

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Reverse Genetics for Mammalian Orthoreovirus

    • Johnasha D. Stuart, Matthew B. Phillips, Karl W. Boehme
    Pages 1-10

  3. Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus

    • Udeni B. R. Balasuriya, Jianqiang Zhang
    Pages 11-28

  4. Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus

    • Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, Dongwan Yoo
    Pages 29-46

  5. Reverse Genetics of Zika Virus

    • Chao Shan, Xuping Xie, Pei-Yong Shi
    Pages 47-58

  6. Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Preemergent Infectious Coronaviruses

    • Adam S. Cockrell, Anne Beall, Boyd Yount, Ralph Baric
    Pages 59-81

  7. Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

    • Erica Bickerton, Sarah M. Keep, Paul Britton
    Pages 83-102

  8. Rescue of Sendai Virus from Cloned cDNA

    • Shringkhala Bajimaya, Tsuyoshi Hayashi, Toru Takimoto
    Pages 103-110

  9. BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)

    • Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore
    Pages 111-124

  10. Recovery of a Paramyxovirus, the Human Metapneumovirus, from Cloned cDNA

    • B. G. van den Hoogen, R. A. M. Fouchier
    Pages 125-139

  11. Reverse Genetics of Newcastle Disease Virus

    • Stivalis Cardenas-Garcia, Claudio L. Afonso
    Pages 141-158

  12. Reverse Genetics Systems for Filoviruses

    • Thomas Hoenen, Heinz Feldmann
    Pages 159-170

  13. Rapid Reverse Genetics Systems for Rhabdoviruses: From Forward to Reverse and Back Again

    • Tobias Nolden, Stefan Finke
    Pages 171-184

  14. Lassa Virus Reverse Genetics

    • Luis Martínez-Sobrido, Slobodan Paessler, Juan Carlos de la Torre
    Pages 185-204

  15. Reverse Genetics of Influenza B Viruses

    • Aitor Nogales, Daniel R. Perez, Jefferson Santos, Courtney Finch, Luis Martínez-Sobrido
    Pages 205-238

  16. Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA

    • Daniela Toro-Ascuy, Marcelo Cortez-San Martín
    Pages 239-250

  17. Plasmid-Based Reverse Genetics of Influenza A Virus

    • Daniel R. Perez, Matthew Angel, Ana Silvia Gonzalez-Reiche, Jefferson Santos, Adebimpe Obadan, Luis Martinez-Sobrido
    Pages 251-273

  18. Back Matter

    Pages 275-282
    Download chapter PDF
Back to top

Editors and Affiliations

  • Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, USA

    Daniel R. Perez

Bibliographic Information

  • Book Title: Reverse Genetics of RNA Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel R. Perez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6964-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6962-3Published: 16 May 2017

  • Softcover ISBN: 978-1-4939-8353-7Published: 24 June 2018

  • eBook ISBN: 978-1-4939-6964-7Published: 15 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 20 b/w illustrations, 32 illustrations in colour

  • Topics: Human Genetics

Publish with us

Policies and ethics

Back to top