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Metastasis

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ulrike S. Stein

    1. Translational Oncology of Solid Tumors Experimental and Clinical Research Center, Charité – Universitätsmedizin Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2294)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Metastasis Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Cancer Cell Invasion and Metastasis in Zebrafish Models (Danio rerio)

      • Sarah Martinez Roth, Eric B. Berens, Ghada M. Sharif, Eric Glasgow, Anton Wellstein
      Pages 3-16

    3. The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells

      • Jörg H. Leupold, Nitin Patil, Heike Allgayer
      Pages 17-26

    4. In Vitro 3D Models of Tunable Stiffness

      • Elysse C. Filipe, Amelia L. Parker, Antonia L. Cadell, Gretel Major, David R. Croucher, Thomas R. Cox
      Pages 27-42

    5. Patient-Derived Xenografts from Solid Tumors (PDX) for Models of Metastasis

      • Annika Wulf-Goldenberg, Jens Hoffmann, Michael Becker, Bernadette Brzezicha, Wolfgang Walther
      Pages 43-58

    6. Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth

      • Ellina Schulz, Tim Hohmann, Urszula Hohmann, Ralf-Ingo Ernestus, Mario Löhr, Faramarz Dehghani et al.
      Pages 59-77

  3. Functional Mechanisms of Metastasis

    1. Front Matter

      Pages 79-79
      Download chapter PDF

    2. Measuring Mechanical and Adhesive Properties of Single Cells Using an Atomic Force Microscope

      • Tim Hohmann, Faramarz Dehghani
      Pages 81-92

    3. Analyzing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells Under Flow Conditions

      • Camilla Cerutti, Anne J. Ridley
      Pages 93-109

    4. Probing Intravascular Adhesion and Extravasation of Tumor Cells with Microfluidics

      • Naël Osmani, Gautier Follain, Valentin Gensbittel, María Jesús García-León, Sébastien Harlepp, Jacky G. Goetz
      Pages 111-132

    5. Real-Time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay Using the IncuCyte System

      • Dennis Kobelt, Wolfgang Walther, Ulrike S. Stein
      Pages 133-142

    6. Measurement of Metabolites from Migrating Cells

      • Demond Williams, Barbara Fingleton
      Pages 143-150

    7. Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrix

      • Rosa Fontana, Jing Yang
      Pages 151-163

    8. Evaluation of Real-Time In Vitro Invasive Phenotypes

      • René M. DeLosh, Robert H. Shoemaker
      Pages 165-180

    9. Detection of Tumor Cell-Induced Platelet Aggregation and Granule Secretion

      • Svenja Schwarz, Martin Schlesinger, Gerd Bendas
      Pages 181-195

    10. Fatty Acid–Driven Polarization of Suppressive Bone Marrow–Derived Macrophages Including Metabolic and Functional Analysis

      • Hao Wu, Rainer Glauben
      Pages 197-207

    11. From Whole Blood to Isolated Pro-Metastasis Immune Cells: An Ex Vivo Approach to Isolate and Manipulate Immune Cells Contributing to Tumor Metastasis

      • Amany Samir, Aya Aly El Khodiry, Hend M. ElTayebi
      Pages 209-218

  4. Prognostication of Metastasis

    1. Front Matter

      Pages 219-219
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    2. A Bioinformatic Pipeline to Identify Biomarkers for Metastasis Formation from RNA Sequencing Data

      • Mathias Dahlmann, Ulrike S. Stein
      Pages 221-238
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Keywords

About this book

This volume provides the most recent developments and methodologies on metastatic process, formation, and detection.  Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Editors and Affiliations

  • Translational Oncology of Solid Tumors Experimental and Clinical Research Center, Charité – Universitätsmedizin Berlin and Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ulrike S. Stein

Bibliographic Information

  • Book Title: Metastasis

  • Book Subtitle: Methods and Protocols

  • Editors: Ulrike S. Stein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1350-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1349-8Published: 20 March 2021

  • Softcover ISBN: 978-1-0716-1352-8Published: 20 March 2022

  • eBook ISBN: 978-1-0716-1350-4Published: 19 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 348

  • Number of Illustrations: 12 b/w illustrations, 60 illustrations in colour

  • Topics: Cancer Research

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