Buch, Englisch, 562 Seiten, HC runder Rücken kaschiert, Format (B × H): 160 mm x 241 mm, Gewicht: 1146 g
Buch, Englisch, 562 Seiten, HC runder Rücken kaschiert, Format (B × H): 160 mm x 241 mm, Gewicht: 1146 g
ISBN: 978-981-1307-84-3
Verlag: Springer Nature Singapore
The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
Zielgruppe
Graduate
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Enzymologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
- Naturwissenschaften Biowissenschaften Proteinforschung
- Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
Weitere Infos & Material
Part I: Enzyme catalysis - a perspective.- Enzymes -Their place in Biology.- Enzymes - Historical aspects.- Exploiting enzymes – Technology.- On enzyme nomenclature and classification.- Hallmarks of an enzyme catalyst.- Origins of enzyme catalytic power.- Origins of enzyme catalytic power.- Which enzyme uses what trick? Some remarks.- Structure and catalysis: Conformational flexibility and protein motion.- Part II: Enzyme kinetic practice and measurements.- Chemical kinetics – Fundamentals.- Concepts of equilibrium and steady-state.- ES complex and pre-steady-state kinetics.- Principles of enzyme assays.- Good kinetic practices.- Quantification of catalysis and measures of enzyme purity.- Henri-Michaelis-Menten equation.- More complex rate expressions.- Enzyme kinetic data - Collection and analysis.- Part III: Elucidation of Kinetic mechanisms.- Approaches to kinetic mechanism – Overview.- Analysis of initial velocity patterns.- Enzyme inhibition analyses.- Irreversible inhibitions.- Reversible inhibitions.- Alternate substrate (product) interactions.- pH Studies with enzymes.- Isotopes in enzymology.- Isotope exchanges at equilibrium.- Isotope effects in enzymology.- From kinetic data to mechanism and back.- Part IV: Chemical mechanisms and catalysis.- Chemical reactivity and molecular interactions.- Acid-base chemistry and catalysis.- Nucleophilic catalysis and covalent reaction intermediates.- Phosphoryl group chemistry and importance of ATP.- Enzymatic oxidation-reduction reactions.- Carboxylations and decarboxylations.- Electrophilic catalysis and amino acid transformations.- Integrating kinetic and chemical mechanisms – A synthesis.- Part V: Frontiers in enzymology.- Regulation of enzyme activity.- In vitro versus in vivo - Concept and consequences.- Future of enzymology – An appraisal.- Closure - Whither Enzymology.