Buch, Englisch, 253 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Springer Protocols Handbooks
Comprehensive Protocols and Best Practices
Buch, Englisch, 253 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Springer Protocols Handbooks
ISBN: 978-1-0716-5534-4
Verlag: Springer Us
This volume details foundational practices and emerging innovations in microbial biofilm research. Chapters guide readers through in vitro and in vivo biofilm models, imaging and quantitative analysis techniques, molecular and genetic approaches, high-throughput screening strategies, and methods for studying biofilm-host and biofilm-material interactions.
Authoritative and cutting-edge, aims to provide practical guidance and inspire further research and innovation.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Chapter 1-Biofilm Formation and Its Significance in Biological Systems.- Chapter 2-Microscopy-Based Methods for Visualizing Biofilm Architecture and Structure: Light Microscopy.- Chapter 3- Fluorescence Microscopy Techniques for Biofilm Visualization and Quantification.- Chapter 4- Microscopy-Based Methods: Visualizing Biofilm Architecture and Structure with a Focus on Confocal Laser Scanning Microscopy (CLSM).- Chapter 5- Detection and Structural Characterization of Microbial Biofilms Using Scanning Electron Microscopy.- Chapter 6- Detection and Ultrastructural Characterization of Biofilms by Transmission Electron Microscopy.- Chapter 7- Crystal Violet–Based Colorimetric Assay for Biofilm Evaluation.- Chapter 8- Fluorescence-Based Determination of Live and Dead Cells in Biofilm Communities.- Chapter 9- Rapid Colorimetric Methods for Evaluating Biofilm Viability Using Tetrazolium and Resazurin Dyes.- Chapter 10- Polymerase Chain Reaction at the Biofilm Interface: Tools for Detection, Quantification, and Analysis.- Chapter 11-Characterization of Biofilm-Associated Microbial Communities Using 16S rRNA Gene Sequencing.- Chapter 12- Metagenomic Approaches for the Detection and Characterization of Biofilms.- Chapter 13-Transcriptomics (RNA-seq): Exploring and Revealing Gene Expression Patterns During Biofilm Formation.- Chapter 14- Real-Time Monitoring of Microbial Biofilms Using Quartz Crystal Microbalance.- Chapter 15- Atomic Force Microscopy Approaches for Biofilm Investigation.- Chapter 16- Flow Cell Systems: Real-Time Observation of Biofilm Formation Under Controlled Flow Conditions.- Chapter 17- Culture-Based Quantification of Biofilms Using Swab Sampling and CFU Enumeration.- Chapter 18- Rapid Detection of Biofilms Using Fourier Transform Infrared Spectroscopy.- Chapter 19- A Stepwise Approach to Biofilm Detection Using Nuclear Magnetic Resonance.- Chapter 20- Biofilm Detection and Profiling Using MALDI-TOF and Liquid Chromatography–Mass Spectrometry.
