Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer

Erscheinungsjahr 2026
ISBN: 978-0-443-43120-3
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models, Investigation of the in vitro lytic capacity of CAR-T by microscopyal, Antigen-specific T cell cytotoxicity toward target tumor cells, In vitro expansion of stem-like memory T cells via D-mannose supplementation, and much more.

Additional chapters explore High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells, Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation, Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry, Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy, ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS, Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry, and much more.

Autoren/Hrsg.

Weitere Mitwirkende

Thomas, Clément

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie

Inhaltsverzeichnis

1. An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol
Emmanuel intégrative des tumeurs et immunothérapie Donnadieu, Irena Rajnpreht and Lene Vimeux
2. new protocol to measure mitochondria polarization/function at the immunological synapse
Manuel Izquierdo, Javier Ruiz Navarro and Victor Calvo
3. Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models
Teresa Lozano, Noa Beatriz Martín-Cófreces, Álvaro Gómez-Morón and Celia Martín-Otal
4. Investigation of the in vitro lytic capacity of CAR-T by microscopy'
Noa Beatriz Martín-Cófreces, Teresa Lozano, Álvaro Gómez-Morón and Celia Martín-Otal
5. Antigen-specific T cell cytotoxicity toward target tumor cells
Chong Sun and Helena Engel
6. In vitro expansion of stem-like memory T cells via D-mannose supplementation
Bo Huang, Guideng Li, Yajing Qiu and Cngcong Zhao
7. High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells.
Hélène Sophie DANIELS-TREFFANDIER, Loic Dupre, Beatriz CHAVES and Nicolas SOCQUET-JUGLARD
8. Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation
Yu Cheng, Rui Gao, Wanxin Huang and Yehe Kang
9. Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry
Massimo Vitale, Paola Orecchia, Guido Ferlazzo, Monica Parodi, Simonetta Astigiano, Valentina Delli Zotti, Filippo Piombo, Stefania Martini, Simona Pigozzi and Paolo Carrega
10. Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy.
Charles Daniel Murin, Emily Mace, Patrick Ross, Jessica Matthias, Daniel Leaman, Micahel Zwick and Scott Henderson
11. Assessment of NK cell cytotoxicity induced by il-15 based immunotherapy against cancer cells
Carole Seguin-Devaux, Camille Rolin, Gilles Iserentant and Aubin Pitiot
12. Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry
Sophie-Christin Linkenbach, Ayline Kübler and Tommaso Torcellan
13. Anti-tumor activity characterization of NK cell subsets by flow cytometry
Massimo Vitale, Carola Perrone, Paolo Canevali, Veronica Colamassaro, Elena Giusto, Silvia Tamburini, Gianluca Ubezio, Guido Ferlazzo and Raffaella Meazza
14. DNA Origami-Decorated Lipid Bilayer Systems for Interrogating the Spatial Requirements underlying B cell Immune-Synapse Formation
Bebhinn Treanor, Yuchen Hou, Chung -Yi Tseng, Raysa Orin, Emily Cohen and Leo Chou

Über Autor(innen)

Thomas, Clément
Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.