Buch, Englisch, 464 Seiten
Buch, Englisch, 464 Seiten
ISBN: 978-1-264-87694-5
Verlag: McGraw Hill LLC
Learn to get the most out of your molecular biology experimentsExperimental Techniques in Molecular Biology is a comprehensive, hands-on resource designed to equip students and researchers with the essential tools and knowledge to explore biology at the molecular level. This unique guide demonstrates how to identify and characterize organisms, locate genes or disease-causing agents, and apply molecular insights to real-world biological challenges.Covering a wide range of laboratory techniques essential to recombinant DNA technology, this book brings together everything you need in one accessible volume. You’ll learn how to target nucleic acids—both single- and double-stranded RNA and DNA—as well as proteins from diverse organisms.Step-by-step instructions guide you through preparing solutions and buffers, and extracting RNA, dsRNA, DNA, and proteins from virus-infected plant tissues, fungi, blood, bacteria, nematodes, and insects and other animals.The book thoroughly explains techniques for isolation, characterization, quantification, and sequencing. Each method is presented with clear background information, a detailed rationale, and a breakdown of the reagents used and their functions.Perfect for both the classroom and the laboratory, this guide empowers you to confidently perform and understand the molecular techniques at the core of today’s bioscience research.• Features lab techniques not present in other core textbooks• Includes 100 full-color illustrations for visual clarity• Authored by a seasoned academic with extensive teaching and research experience
