Buch, Englisch, Format (B × H): 152 mm x 229 mm
Buch, Englisch, Format (B × H): 152 mm x 229 mm
ISBN: 978-0-443-52233-8
Verlag: Elsevier Science & Technology
This volume of Methods in Enzymology, entitled “Arginase: Structure, Function, and Therapeutic Targeting,” provides a comprehensive, methodology-driven perspective on arginase research spanning molecular, biochemical, and translational domains.
The volume is organized into four sections that reflect the breadth of the field, spanning structural and mechanistic enzymology, inhibitor design and therapeutic development, clinical and metabolic aspects of arginine biology, and the evolutionary and functional diversity of arginase and related enzymes. Chapters cover experimental approaches to enzyme expression, purification, and structural characterization; synthetic and computational strategies for inhibitor discovery; and emerging therapeutic modalities, including antibody-based targeting of specific arginase isoforms, advanced delivery systems, and engineered enzyme formulations.
Importantly, the volume extends beyond classical views of arginase as an enzyme of arginine metabolism, highlighting its broader structural and functional context within the ureohydrolase superfamily and related guanidinohydrolase enzymes, including alternative substrates, catalytic diversity, and roles in microorganisms and plants. These perspectives emphasize arginase function in nitrogen metabolism, host–pathogen interactions, and environmental and physiological adaptation.
By integrating contributions from both academia and industry, this volume emphasizes reproducible methodologies and provides a cohesive “from enzyme to organism” framework, connecting fundamental structure–function relationships with applications in metabolism, vascular biology, and therapeutic development.
Fachgebiete
Weitere Infos & Material
Section I: Molecular and Structural Approaches to Arginase Function and Inhibition
1. Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank
E. D'Antonio, Luigi Franklin Di Costanzo and David W. Christianson
2. Synthesis of First-Generation Arginase Inhibitors
F. Sodano
3. Computational and Structure-Guided Design of Arginase Inhibitors
M.A. Fumagalli
4. Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis
George Georgiou
Section II: Advanced Therapeutic Strategies and Analytical Methods for Arginase
5. Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems
L. Sloan and M. Rudebeck
6. Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization
M. Grzybowski
7. Synthesis of Second-Generation Arginase Inhibitors
F. Sodano
8. Cryo-EM Workflow and Structural Characterization of Arginase–Antibody Complexes
G. Scapin
9. Development and Validation of an Electrochemical Assay for Arginase Activity
A. Miglione, Luigi Franklin Di Costanzo and Stefano Cinti
Section III: Clinical, Metabolic, and Translational Aspects of Arginase
10. Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion
A.K. Sau
11. Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives
J. French
12. Arginase Deficiency and Urea Cycle Disorders: Clinical and Metabolic Management
N. Brunetti-Pierri
13. Inborn Errors of Metabolism and Arginine Pathways: Experimental and Clinical Approaches
A. Schulze
14. Arginine Metabolism and Protein Balance: Experimental Approaches in Human Physiology
M. P. K. J. Engelen
15. Arginase in Endothelial Dysfunction and Vascular Disease: Experimental and Translational Approaches
W. Caldwell and R. Caldwell
16. Arginase in Cardiovascular and Vascular Inflammatory Disease
W. Durante
Section IV: Evolutionary and Functional Diversity of Arginase and Related Enzymes
17. Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB
Y. Matoba and Kosuke Oda
18. Beyond Arginase: Structural Biology of the Extended Guanidinase Family
G. Mlynek, S. V. Kundapura and D. Carugo
19. Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism
