Oxidative Stress Biomarkers and Antioxidant Protocols

1. Auflage. Softcover version of original hardcover Auflage 2002
ISBN: 978-1-61737-222-3
Verlag: Springer

Buch, Englisch, Band 186, 322 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 494 g

Reihe: Methods in Molecular Biology

The first protocols book, Free Radical and Antioxidant Protocols (1) was published in late 1998. Sections were divided into three parts, covering selected biochemical techniques for measuring oxidative stress, antioxidant (AOX) activity, and combined applications. In choosing the 40 methods to be included in that book, I realized there were considerably more of equal value than that which we could have presented in a single volume. To produce a comprehensive resource, this book and a third are being compiled to expand coverage of the field. A summary of papers (2) published on this important subject emphasizes the continuing rapid growth in oxidative stress investigations relating to our understanding of biochemical reactions, their relevance to pathophysiological mechanisms, how disease may arise, and how therapeutic intervention may be achieved(3). Although there is some overlap between the categories, the ana- sis shown below illustrates where current studies are concentrated and are almost evenly distributed between free radicals and AOX. Over the last 4 yr, there has been a 55% increase in the number of papers published in the area.

Zielgruppe

Research

Autoren/Hrsg.

Armstrong, Donald

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Techniques for Free Radical Derived Biomarkers.- Human Xanthine Oxidoreductase Determination by a Competitive ELISA.- Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC.- Determination of Products of Lipid Oxidation by Infrared Spectroscopy.- Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry.- Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies.- Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes.- Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidant Stress.- Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein.- Detection of Certain Peroxynitrite-Induced DNA Modifications.- Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS.- Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins.- Rapid Determination of Glutamate Using HPLC Technology.- A Rapid Method for the Quantification of GSH and GSSG in Biological Samples.- Protein Carbonyl Measurement by ELISA.- N ?-(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins.- Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis.- Oxidation of Cellular DNA Measured with the Comet Assay.- Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis.- Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA.- Techniques for Antioxidant Biomarkers.- Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices.-Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode.- Measurement of ?-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry.- Analysis of Tocotrienols in Different Sample Matrixes by HPLC.- Measurement of ?-Carotene15,15?-Dioxygenase Activity by Reverse-Phase HPLC.- Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress.- Catechol- and Pyrogallol-Type Flavonoids.- Pyruvate Dehydrogenase Complex as a Marker of Mitochondrial Metabolism.- Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity.- Metallothionein Determination by Isocratic HPLC with Fluorescence Derivatization.- Quantification of Oxidized Metallothionein by a Cd-Saturation Method.- Fractionation of Herbal Medicine for Identifying Antioxidant Activity.- Designing Safer (Soft) Drugs by Avoiding the Formation of Toxic and Oxidative Metabolites.- Statistical Correction of the Area Under the ROC Curve in the Presence of Random Measurement Error and Applications to Biomarkers of Oxidative Stress.