Buch, Englisch, Band 844, 296 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 780 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 844, 296 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 780 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-526-8
Verlag: Humana Press
The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
1. ENU Based Phenotype Driven Screening
Vera M. Ripoll, Philip L. Kong, and Paul K. Potter
2. Detection and Quantification of Cytokines and Other Biomarkers
Evan L. Chiswick, Elizabeth Duffy, Brian Japp and Daniel Remick
3. Flow Cystometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoetic Stem and Progenitor Cells
Valérie Barbier, Bianca Nowlan, Jean-Pierre Lévesque and Ingrid G. Winkler
4. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo
Valérie Barbier, Ingrid G. Winkler, Robert Wadley and Jean-Pierre Lévesque
5. Analyzing Cell Death Events in Cultured Leukocytes
Karin Christenson, Fredrik B. Thorén and Johan Bylund
6. Towards a Four-Dimensional View of Neutrophils
Ben A. Croker, Andrew W. Roberts and Nicos A. Nicola
7. Isolation of Human and Mouse Neutrophils Ex vivo and In vitro
Yan Hu
8. Measurement of Oxidative Burst in Neutrophils
Yu Chen and Wolfgang G. Junger
9. Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic SubstratesBrice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano and Francis Gauthier
10. The Macrophage
C.P. Verschoor, A. Puchta and D.M.E. Bowdish
11. Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice
R. Tedjo Sasmono and Elizabeth Williams
12. Generation of Mouse Bone Marrow-derived Macrophages
Silvia Manzanero
13. Isolation and Differentiation of Monocytes-macrophages from Human Blood
Dipti Vijayan
14. In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
André Moraes Nicola and Arturo Casadevall
15. Measuring the Inflammasome
Olaf Groß
16. Arginine and Macrophage Activation
Mònica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas and Antonio Celada
17.Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation
Catherina H. Bird,Corrine Hitchen, Mark Prescott, Ian Harper and Phillip I. Bird
18. Detection of Human and Mouse Granzyme B Activity in Cell Extracts
Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird and Dion Kaiserman
19. T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation
Rajaraman Eri, Michael A. McGuckin and Robert Wadley
20. Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry
Dimitrios Tsikas, Maria-Theresia Suchy, Anja, Bibiana Beckmann and Frank-Mathias Gutzki
