Bacteriophage Therapy

From Lab to Clinical Practice
Softcover Nachdruck of the original 1. Auflage 2018
ISBN: 978-1-4939-8472-5
Verlag: Springer

Buch, Englisch, 303 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 598 g

Reihe: Methods in Molecular Biology

This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practiceaims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Azeredo, Joana

Sillankorva, Sanna

Fachgebiete

• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Bakteriologie

Inhaltsverzeichnis

Part I: Isolation of Bacteriophages

1. Isolation of Bacteriophages for Fastidious Bacteria

Shigenobu Matsuzaki, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Takako Ujihara, and Masanori Daibata

 

2. Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides

Cristina García-Aljaro, Maite Muniesa, and Juan Jofre

3. Isolation of Bacteriophages for Clinically Relevant Bacteria

Sanna Sillankorva

Part II: Characterization of Bacteriophages

4. In Vitro Activity against Planktonic and Biofilm Populations Assessed by Flow Cytometry

Luís Melo, Diana Priscila Pires, and Joana Azeredo

5. Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy  

Ana Cuervo and José L. Carrascosa

6. Bacteriophage Taxonomy: an Evolving Discipline

Igor Tolstoy, Andrew M. Kropinski, and J. Rodney Brister

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Part III: Bacteriophage Selection and Cocktail Formulation

7. Determination of the Bacteriophage Host Range – Culture Based Approach

Andrey V. Letarov, and Eugene E. Kulikov

8. Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage

Libera Latino and Christine Pourcel

9. Guidelines to Compose an Ideal Bacteriophage Cocktail

Maia Merabishvili, Jean-Paul Pirnay, and Daniel De Vos

 

Part IV: Biodistribution, Host Interaction and Clinical Application

10. Interaction of Bacteriophages with Mammalian Cells

Zuzanna Kazmierczak and Krystyna Dabrowska

11. In Vivo Bacteriophage Biodistribution

Nicolas Dufour, Raphaëlle Delattre, and Laurent Debarbieux

12. Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-specific Antibodies

Krystyna Dabrowska

&

nbsp; 13. Bacteriophage Treatment of Infected Diabetic Foot Ulcers

Vera V. Morozova, Yulia N. Kozlova, Denis A. Ganichev, and Nina V. Tikunova

14. Compassionate Use of Bacteriophage Therapy for Foot Ulcer Treatment as an Effective Step for Moving Toward Clinical Trials

Randolph Fisha, Elizabeth Kutter, Gordon Wheat, Bob Blasdel, Mzia Kutateladze, and Sarah Kuhl

Part V: Production, Purification and Bacteriophage Storage

15. Bacteriophage Production in Bioreactors

Maryam Agboluaje and Dominic Sauvage

16. Computational Modelling of Bacteriophage Production for Process Optimization

Konrad Krysiak-Baltyn and Sally L. Gras

17. Methods for Bacteriophage Preservation

Malgorzata B. Lobocka, Aleksandra Glowacka, and Piotr Golec

Part VI: Safety and Regulation

18. Bacteriophage Production in Compliance with Regulatory

Requirements

Jean-Paul Pirnay, Maia Merabishvili, Hilde Van Raemdonck, Daniel De Vos, and Gilbert Verbeken

19. Guidelines for Bacteriophage Product Certification

Alan Fauconnier

Part VII: New Phage Therapy Approaches

20. Nano/micro Formulations for Bacteriophage Delivery

Pilar Cortés, Mary Cano-Sarabia, Joan Colom, Jennifer Otero, Daniel Maspoch, and Montserrat Llagostera

21. Synthetic Biology to Engineer Bacteriophage Genomes

Ana Rita Costa, Catarina Milho, Joana Azeredo, and Diana Priscila Pires