Buch, Englisch, Band 761, 295 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 779 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 761, 295 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 779 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-181-9
Verlag: Humana Press
To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
1. Overview of Cell Synchronization
Gaspar Banfalvi
2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Gaspar Banfalvi
3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells
Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer
4. Large Scale Mitotic Cell Synchronization
Kalyan Dulla and Anna Santamaria
5. Synchronization of Mammalian Cell Cultures by Serum Deprivation
Thomas J. Langan and Richard C. Chou
6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry
Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka
7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes
Takashi Iwamatsu
8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
Congjun Li
9. Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover during the Cell Cycle
Jennifer A. Fischer and Salvatore Caradonna
10. Synchronization of HeLa Cells
Hoi Tang Ma and Randy Y.C. Poon
11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth
Gaspar Banfalvi
12. Synchronization of Yeast
Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider
13. Synchronization of Pathogenic Protozoans
Staffan Svärd and Karin Troell
14. Synchronization of In VitroMaturation in Porcine Oocytes
Tamas Somfai and Yuji Hirao
15. Synchronization of Medicago sativa Cell Suspension Culture
Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth
16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)
Yoel Shufaro and Benjamin E. Reubinoff
17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, and Alla Dolnikov
18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients
Fabienne Venet, Caroline Guignant, and Guillaume Monneret
19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish
Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky
