Bioorthogonal Labeling

Erscheinungsjahr 2026
ISBN: 978-0-443-43224-8
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Biorthogonal Tagging, Volume 731 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters such as Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology, Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids, Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation, Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications, 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs, Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation, and much more.

Additional chapters cover Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles, Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions, Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry, Bioorthogonal Tagging of DNA and RNA, Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes, and many more timely topics.

Autoren/Hrsg.

Weitere Mitwirkende

Christianson, David

Allen, Karen N.

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology
Stefan Bräse, Juliane Ott and Ute Schepers
2. Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids
Frederic Friscourt and Zoeisha Chinoy
3. Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation
Helma Wennemers
4. Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications
John Paul Pezacki
5. 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs
Yu-Hsuan Tsai, Linlin Wang, Gaocan Dai and Sicong Yao
6. Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation
Yu-Hsuan Tsai, Yawei Liu, Lei Zhang and Sicong Yao
7. Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles
Maoliln Lu
8. Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions
Maoliln Lu
9. Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry
Danielle H. Dube, Aniqa Chowdhury, Divya Bhargava and Karen Moulton
10. Bioorthogonal Tagging of DNA and RNA
Seergazhi G. Srivatsan
11. Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes
Catherine L. Grimes
12. Dendritic Cell Metabolic Labeling and Targeting
Hua Wang
13. Orthogonal Crosslinking of Monobodies Using a Genetically Encoded ß-Lactam Amino Acid
Qing Lin
14. Bioorthogonal Chemical Labeling of Endogenous Receptors in the Live Mouse Brain
Itaru Hamachi
15. Organelle-Directed Metabolic Glycan Labeling
Shou-Fa Han, Shixiong Wen, Yilong Shi, Enkang Zhang and Jiahuai Han
16. Bioorthogonal Click Labeling of Proteins for Molecular Resolution Fluorescence Imaging
Markus Sauer
17. Molecular Engineering of Activatable Cyclopropenes for Bioorthogonal Labeling
Scott T. Laughlin
18. Pyrone and Pyranthione Scaffolds for Click-to-Release Biological Gas Delivery
Scott T. Laughlin
19. Click and Release through Bioorthogonal Hydroamination
Justin Kim, Tyler J Brown and Yiming Guo
20. Site-Specific Bioorthogonal Labeling of Antibodies
Lai-Xi Wang
21. inCu-click: Enabling Copper-Catalyzed Click Chemistry Inside Living Cells
Sara H. Rouhanifard
22. Bioorthogonal Photocatalytic Proximity Labeling for Quantitative Mapping of Cell–Cell Interactions
Xinyuan Fan, Yan Zhang, Nan Zhou and Fuhu Guo
23. Bioorthogonal Labeling of Sialic Acid Isomers for Imaging of Glycoconjugates Using Mass Spectrometry and Microscopy
Richard R. Drake

Über Autor(innen)

Allen, Karen N.
Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Christianson, David
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.