DNA Repair Protocols

3rd Auflage 2012
ISBN: 978-1-61779-997-6
Verlag: Humana

Buch, Englisch, Band 920, 631 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1404 g

Reihe: Methods in Molecular Biology

Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology ? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Bjergbæk, Lotte

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Naturwissenschaften Biowissenschaften Humanbiologie

Inhaltsverzeichnis

1. DNA repair: Front and Center and Not Going Away!
Vilhelm A. Bohr

Part I. Identification of Repair Genes and Generation of Repair Mutants

2. Identification of Genes Required for Damage Survival using a Cell Based RNAi Screen against the Drosophila Genome
Dashnamoorthy Ravi and Alexander James Roy Bishop

3. Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans using Genome-wide RNAi Screening

Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard and Anders Olsen.

4. Establishment of the DNA Repair-defective Mutants in DT40 Cells
Masamichi Ishiai, Emi Uchida, and Minoru Takata

Part II. Assessing DNA Damage and Repair

5. Electrophoretic Mobility Shift Assays for
Protein-DNA Complexes Involved in DNA Repair
Chun Tsai, PhD, Vaughn Smider, Byung Joon Hwang,  and Gilbert Chu

6. The Comet Assay A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Rothfuss

7. Detection of DNA Damage by Comet Fluorescence in situ Hybridization (Comet-FISH)

W. Schlörmann and M. Glei

8. Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay
Rui Oliveira and Björn Johansson

9. Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells using Quantitative PCR
Amy M. Furda, Amanda Smith Bess,  Joel N. Meyer, Bennett Van Houten

Part III. Repair of Incorrect or Damaged DNA Bases

10. In vitro DNA Mismatch Repair in Human Cells
Liya Gu, Charles M. Ensor, and Guo-Min Li

11. Eukaryotic DNA Mismatch Repair in vitro
Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang

12.  Immuno-slot Blot Assay for Detection of UVR-mediated DNA Damage
Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke

13.  Quantification of DNA Photoproductions in Mammalian Cell DNAUsing Radioimmunoassay
Thomas R. Berton and David L. Mitchell

14. Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-mediated PCR
Ahmad Besaratinia and Gerd P. Pfeifer

15. Construction of Plasmids Containing Site-specific DNA Interstrand Crosslinks for Biochemical and Cell Biological Studies
Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schärer

16. Replication-coupled DNA Interstrand Crosslink Repair in  Xenopus Egg Extracts
Puck Knipscheer, Markus Räschle, Orlando D. Schärer, and Johannes C. Walter

17.  In Vitro Base Excision Repair using Mammalian Cell Extracts
Jason L. Parsons and Grigory L. Dianov,

18.  Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage in vitro
Dolores Córdoba-Cañero, Teresa Roldán-Arjona and Rafael R. Ariza

19.  In vitro monitoring of Base Excision Repair in Saccharomyces cerevisiae
Simone Moertl and Fred Ahne

20. Mitochondrial Base Excision Repair Assays
Ricardo Gredilla and Tinna Stevnsner

Part IV. DNA Strand Breakage and Repair

21. O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks
April M. Luke and Jun Nakamura

22.  DNA Double Stand Break Damage and Repair Assessed by Pulse-field Gel

Electrophoresis
Helen E. Bryant

23. Quantitative Amplification of Single Stranded DNA (QAOS)

Eva-Maria Holstein and David Lydall

24. Simple, Non-radioactive Measurement of Single-stranded DNA at Telomeric, Sub-telomeric and Genomic Loci in Budding Yeast
James M Dewar and David Lydall

25. Monitoring DNA Recombination Initiated by HO Endonuclease
Neal Sugawara and James E. Haber

26. Monitoring I-SceI Induced Double Strand Break Repair in DT40 Cells
David du Plessis and Julian E. Sale

27. I-SceI-based Assays to Examine Distinct Repair Outcomes of Mammalian Chromosomal Double Strand Breaks
Amanda Gunn and Jeremy M. Stark

28. Studying Repair of a Single Protein-bound Nick in vivo using the Flp-nick System
Ida Nielsen, Anni H. Anderse