Buch, Englisch, 502 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 502 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5746-1
Verlag: Springer Us
This fully updated volume explores experimental approaches used to investigate phosphoinositide (PtdInsP) regulation and function, featuring updates as well as new emerging methods and technologies. The book collects methods to identify and quantify the levels of each PtdInsP species, bioluminescence-generating tools, super-resolution, and optogenetics techniques, as well as methods to disrupt PtdInsP kinases and phosphatases. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, serves as an ideal guide to complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.
Zielgruppe
Popular/general
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling.- Profiling of Phosphoinositide Molecular Species in Resting or Activated Human or Mouse Platelets by a LC-MS Method.- Exploring Phosphoinositide Binding Using Native Mass Spectrometry.- Identification of Phosphoinositides by Shotgun Tandem Mass Spectrometry Through Specific Fragment Ions.- Quantification of Genetically Encoded Lipid Biosensors.- Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates.- Staining for Phosphoinositides in Fixed and Permeabilized Cells Using Recombinant Biosensors.- Monitoring Plasma Membrane PI(4,5)P2 Replenishment During Receptor Stimulation in Live Cells.- Detection of Plasma Membrane and Endomembrane Phosphoinositide Dynamics Using Genetically Encoded Fluorescent Protein Probes.- Resolving the Role of Phosphoinositides in Cell Migration.- Quantifying Organelle-Specific Membrane Lipid Dynamics at the Cell Population Scale Using Bioluminescence Resonance Energy Transfer-Based Biosensors.- Super-Resolution Imaging of PI(4,5)P2 and Other Phosphoinositides in the Cell Plasma Membrane with PALM and dSTORM.- Cell-Wide and Subcellular Optogenetic Control of PI(4,5)P2 in Insulin-Secreting Cells: Molecular Engineering and Implementation.- In Situ Detection of Phosphoinositide-Protein Interactions Using Fluorescence Proximity Ligation Assay.- Double-Fluorescent Immunoblot Detection of Phosphoinositide-Protein Complexes.- Characterization of Phosphoinositide-Protein Complexes via Radioactive Inositol Labeling.- Characterization of Protein-Phospholipid/Membrane Interactions Using a “Membrane-on-a-Chip” Microfluidic System.- Liposome Flotation Assay to Study Protein-Phosphoinositide Interactions.- Protein-Lipid Interaction by Fluorescence (PLIF) for Screening Phosphoinositide-Binding Proteins.- Membrane-Dependent Phospholipase Activity of the PLC-g Isozymes Measured with XY-69 and WH-15.
