Atomic Force Microscopy in Biomedical Research

Methods and Protocols
1. Auflage 2011
ISBN: 978-1-61779-097-3
Verlag: Humana Press

Buch, Englisch, Band 736, 408 Seiten

Reihe: Methods in Molecular Biology

Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection. Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.

Zielgruppe

Research

Autoren/Hrsg.

Braga, Pier Carlo

Ricci, Davide

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren
• Naturwissenschaften Biowissenschaften Mikrobiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien

Inhaltsverzeichnis

Part I. The Basics of Atomic Force Microscopy

How the Atomic Force Microscope Works
Davide Ricci and Pier Carlo Braga

Imaging Methods in Atomic Force Microscopy
Davide Ricci and Pier Carlo Braga

Recognizing and Avoiding Artifacts in AFM Imaging
Davide Ricci and Pier Carlo Braga

Advanced Biosensing Using Micromechanical Cantilever Arrays
Martin Hegner and Youri Arntz

Part II. Morphostructural Analysis of Cellular Structures

Analysis of Human Fibroblasts by Atomic Force Microscopy
Gillian R. Bushell, Colm Cahill, Sverre Myhra, and Gregory S. Watson

Corneal Tissue Observed by Atomic Force Microscopy
Stylliani Lydataki, Miltiadis K. Tsilimbaris, Eric S. Lesniewska, Alain Bron, and Iannis G. Pallikaris

AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation
Hiroko Takano and Kazuhiro Abe

Calculation of Cuticle Step Heights from AFM Images of Outer Surfaces of Human Hair
James R. Smith

Imaging Living Chondrocyte Surface Structures With AFM Contact Mode
Gerlinde Bischoff, Anke Bernstein, David Wohlrab, and Hans-Joachim Hein

Growth Cones of Living Neurons Probed by Atomic Force Microscopy
Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco

Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM
Grayson W. Marshall, Jr., Sally J. Marshall, Mehdi Balooch, and John H. Kinney

Applying Atomic Force Microscopy to Studies in Cardiac Physiology
Jason J. Davis, Trevor Powell, and H. Allen O. Hill

Imaging Bacterial Shape, Surface, and Appendages Before and After Treatments With Antibiotics
Pier Carlo Braga and Davide Ricci

Part III. Subcellular Structures Investigation

Visualizing Nuclear Structure In Situ by Atomic Force Microscopy
Luis Felipe Jiménez-García and María de Lourdes Segura-Valdez

Imaging Surface and Submembranous Structures in Living Cells With the Atomic Force Microscope: Notes and Tricks
Filip Braet and Eddie Wisse

Atomic Force Microscopy of Protein Complexes
Olga I. Kiselyova and Igor V. Yaminsky

Atomic Force Microscopy of Interfacial Monomolecular Films of Pulmonary Surfactant
Kaushik Nag, Robert R. Harbottle, Amiyo K. Panda, and Nils O. Petersen

High-Resolution Analysis of the 3D Organization of Human Metaphase Chromosomes
Stefan Thalhammer, Pietro Gobbi, Mirella Falconi, Giovanni Mazzotti, and Wolfgang M. Heckl

Shape and Volume of Living Aldosterone-Sensitive Cells Imaged With the Atomic Force Microscope
Stefan W. Schneider, Rainer Matzke, Manfred Radmacher, and Hans Oberleithner

Localization of Epithelial Sodium Channels by Atomic Force Microscopy
Peter R. Smith and Dale J. Benos

High-Resolution Imaging of Bacteriorhodopsin by Atomic Force Microscopy
Dimitrios Fotiadis and Andreas Engel

Part IV. Functional Investigations With AFM

Measurement of Mechanical Properties of Intact Endothelial Cells in Fresh Arteries
Hiroshi Miyazaki and Kozaburo Hayashi

Observation of Oxidative Stress on Yeast Cells
Ricardo de Souza Pereira

Lymphoblastoid Cells Exposed to Low-Frequency Magnetic Fields: Study by Atomic Force Microscopy
Settimio Grimaldi, Marco Girasole, and Antonio Cricenti

Sample Preparation Method for Observing RNA Polymerase Activity by Atomic Force Microscopy
Sandor Kasas

Atomic Force Microscopy of b-Amyloid: Static and Dynamic Studies of Nanostructure and Its Formation
Justin Legleiter and Tomasz Kowalewski

How to Build Up Biosensors With the Cantilever of the Atomic Force Microscope
Ricardo de Souza Pereira

Measurement of Single Molecular Interactions by Dynamic Force Microscopy
Martin Hegner, Wilfried Grange, and Patricia Bertoncini

Index