Buch, Englisch, Band 2750, 193 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 591 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2750, 193 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 591 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-07-163604-6
Verlag: Springer US
Authoritative and cutting-edge, Alpha-1 Antitrypsin: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Introduction: Alpha-1 Antitrypsin Deficiency.- Approaches to Therapeutic Gene Editing in Alpha-1 Antitrypsin Deficiency.-Manipulation of Proteostasis Networks in Transgenic ZAAT Zebrafish via CRISPR-Cas9 Gene Editing.- PCR Analysis to Identify AAT Gene Promoters and Splice Variants.- Native and Ion Mobility Mass Spectrometry Characterization of Alpha-1 Antitrypsin Variants and Oligomers.- Sanger and Next Generation Sequencing of AAT.- Measuring the Concentration of Active Alpha-1 Antitrypsin in Serum.- Characterization of Novel Alpha-1 Antitrypsin Coding Variants in a Mammalian Cellular Model.- Alpha-1 Antitrypsin (A1AT) Proteotyping by LC-MS/MS.- Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-Human Primates and Mice.- An Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Circulating Pi*Z Alpha-1 Antitrypsin Polymers.- Measuring of Alpha1 Antitrypsin Concentration by Nephelometry or Turbidimetry.- Autoantibody Recognition of Natural and Homocysteinylated Alpha-1 Antitrypsin: Indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) Quantification in Sera.- Monitoring the Secretion and Activity of Alpha-1 Antitrypsin in Various Mammalian Cell Types.- Lipoproteins in Negative Feedback with Alpha-1 Antitrypsin.- Silencing Very Low Density Lipoprotein Receptor Reveals Alpha-1 Antitrypsin Role in HIV infectivity.- Purified versus Plasma Alpha-1 Antitrypsin Effects on Cellular Activities.