Plant Cell Division

Methods and Protocols
2. Auflage 2022
ISBN: 978-1-0716-1743-4
Verlag: Springer US

Buch, Englisch, Band 2382, 267 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 729 g

Reihe: Methods in Molecular Biology

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Caillaud, Marie-Cécile

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Botanik

Inhaltsverzeichnis

1. Methods to Visualize the Actin Cytoskeleton during Plant Cell Division

            Marie-Cécile Caillaud

Part I: Mitosis and Cell Cycle Progression

2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

            Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, and Inna Lermontova

3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips

            Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, and William F. Thompson

4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures

            Katerina Bišová

5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission

            Veronika Kselíková, Vilém Zachleder, and Katerina Bišová

Part II: Manipulation of Cell Division

6. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy

            Lukas Hoermayer, Jirí Friml, and Matouš Glanc

7. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells

            Tamara Jimenez-G?ngora, Huang Tan, and Rosa Lozano-Durán

Part III: Quantification of Cell Division Patterning

8. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf

            Jingjing Fang and Xueyong Li

 

9. Studying Cell Division Plane Positioning in Early-Stage Embryos

            Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia

10. Means to Quantify Vascular Cell File Numbers in Different Tissues

            Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, and Bert De Rybel

11. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis

            Roya Campos and Jaimie M. Van Norman

12. Quantification of Cell Division Angles in the Arabidopsis Root

            Katia Belcram, David Legland, and Martine Pastuglia

Part IV: Imaging and Quantifying Plant Cytokinesis

13. A Quantitative Method for Evaluating Phragmoplast Morphology

            Takema Sasaki and Yoshihisa Oda

14. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis

            Alexandra Deli, Leda-Eleni Tympa, and Panagiotis N. Moschou

15. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells

            Keisho Maeda and Takumi Higaki

16. Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae

            Chikako Nagasato, Rina Yonamine, and Taizo Motomura