Citrus Tristeza Virus

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9557-8
Verlag: Springer

Buch, Englisch, Band 2015, 247 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 693 g

Reihe: Methods in Molecular Biology

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Catara, Antonino F.

Licciardello, Grazia

Bar-Joseph, Moshe

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Naturwissenschaften Biowissenschaften Botanik Pflanzenpathologie, Pflanzenkrankheiten
• Naturwissenschaften Biowissenschaften Virologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie

Inhaltsverzeichnis

1. A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies

Moshe Bar-Joseph



2. A Brief Historical Account of the Family Closteroviridae

Giovanni P. Martelli



3. Phenotyping Biological Properties of CTV Isolates

Marcella Russo and Antonino F. Catara



4. CTV Vectors and Interactions with the Virus and Host Plants

Raymond Yokomi



5. Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues

Mariano Cambra, Eduardo Vidal, Carmen Martínez, and Edson Bertolini



6. Detection of Citrus Tristeza Virus and Co-Infecting Viroids

Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, and Francesco Di Serio



7. Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP

Elisavet K. Chatzivassiliou and Grazia Licciardello



8. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus

Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, and Raymond Yokomi



9. Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarr