Buch, Englisch, Format (B × H): 152 mm x 229 mm
Buch, Englisch, Format (B × H): 152 mm x 229 mm
ISBN: 978-0-443-42957-6
Verlag: Elsevier Science & Technology
Cell Death: Part F, Volume 211 presents the latest advances and comprehensive reviews in the rapidly evolving field of cell death. This volume explores the molecular mechanisms, regulatory pathways, and biological consequences of various forms of cell death—including apoptosis, necrosis, autophagy, and other non-canonical processes. Chapters in this new release include Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells, Methods to study PIDDOsome and caspase 2 signaling, Isolation, characterization, and experimental applications of apoptotic bodies in vitro, Lipid ROS Measurement by Spectral Flow Cytometry based on the ratiometric dye BODIPYTM C11 581/591, and much more.
Additional chapters cover CRISPR-Cas9 Screen to Identify Genes Regulating Cell Death, Flow cytometry-assisted discrimination of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae, Cytofluorometric analysis of cell death in primary human neutrophils, Real-time tracking of cell death in human intestinal organoids: a quantitative imaging approach, and more.
Fachgebiete
Weitere Infos & Material
1. Methods to Assess BAX Conformation, Activation, Translocation, and Mitochondrial Outer Membrane Permeabilization
Evripidis Gavathiotis and Deniz Gulfem Ozturk
2. Proximity biotin labelling identification of Bcl-2 protein interactions in live cells.
Andrew Gilmore
3. Bimolecular fluorescence complementation assays for investigating Bcl-2 family protein interactions
Isabel Marzo, Andrea Benedí, Oscar Gonzalo, Laura Vela and Javier Naval
4. Assessing apoptotic sensitivity in primary mouse B cells by BH3 profiling
Joel Riley
5. Liposome permeabilisation assays to monitor pore formation by cell death proteins
Peter Czabotar, Michelle S. Miller, Katherine Davies and Ahmad Wardak
6. Flow Cytometry Assessment of Mitochondrial Membrane Potential, Biomass, and ROS in OXPHOS-Dependent and Glycolytic Cancer Cells during Cell Death Induction
Nikita Markov and Hans-Uwe Simon
7. Assessment of Viability Versus Cell Death: What is What?
Boris Zhivotovsky, Gelina S. Kopeina, Mikhail S. Chesnokov, Nikolay V. Pervushin and Maria A. Yapryntseva
8. Flow cytometry-based drug response profiling of primary B-precursor ALL cells in co-culture with bone marrow-derived stromal cells
Klaus Debatin, Julia Zinngrebe, Alexandra Niedermayer and Lüder-Hinrich Meyer
9. An in vitro model of efferocytosis in macrophage foam cells to probe lipid-driven dysregulation of dead cell clearance
Kha Thanh Phan, Thu Uyen Tran, Mark Hulett and Tien Nguyen
10. Measuring tumor cell killing using in vivo bioluminescence imaging
Victor Peperzak
