Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part B

Erscheinungsjahr 2019
ISBN: 978-0-12-818119-5
Verlag: Elsevier Inc

Buch, Englisch, 466 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 792 g

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

Zielgruppe

<p>Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers</p>

Autoren/Hrsg.

Weitere Mitwirkende

Shukla, Arun K.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Enzymologie

Inhaltsverzeichnis

1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems
Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori
2. Design and synthesis of fluorescent activity probes for protein phosphatases
Garrett R. Casey, Jon R. Beck and Cliff I. Stains
3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells
Travis J. Nelson, Jia Zhao and Cliff I. Stains
4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation
Justin Lawrie, Wei Niu and Jiantao Guo
5. Synthesis and screening of bead-displayed combinatorial libraries
Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek
6. Visualizing the brain's astrocytes
Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin
7. Modular activatable bioorthogonal reagents
Pratik Kumar and Scott T. Laughlin
8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors
Sam Lear, Zaid Amso and Weijun Shen
9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes
Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi
10. Selection and optimization of enzyme reporters for chemical cytometry
Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton
11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactions
Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow
12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity
Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu
13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting
Narek Darabedian and Matthew R. Pratt
14. Optogenetic perturbation of the biochemical pathways that control cell behavior
Lauren L. Haar, David S. Lawrence and Robert M. Hughes
15. pH-shift stress on antibodies
Hiroshi Imamura and Shinya Honda
16. Small molecule target identification using photo-affinity chromatography
Seung-Yong Seo and Timothy W. Corson
17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases
Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai
18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells
Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi
19. Bioorthogonal oncometabolite ligation
Chloe A. Briney, Susana Najera and Jordan L. Meier

Über Autor(innen)

Shukla, Arun K.
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.