Carbohydrate Microarrays

Methods and Protocols
2012
ISBN: 978-1-61779-372-1
Verlag: Humana Press

Buch, Englisch, Band 808, 427 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1027 g

Reihe: Methods in Molecular Biology

Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible,Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe. 

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Chevolot, Yann

Fachgebiete

• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik

Inhaltsverzeichnis

1.  Recent Advances and Future Challenges in Glycan Microarray Technology

            José L. de Paz and Peter Seeberger

2. Chemical Synthesis of Carbohydrates and Their Surface Immobilisation: A Brief Introduction

            Daniel B. Werz

3. General Consideration on Sialic Acid Chemistry

            Hongzhi Cao and Xi Chen

4. Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates

            Samy Cecioni, David Goyard, Jean-Pierre Praly, and Sébastian Vidal

5. Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging

            Julia Bartoli, André Roget, and Thierry Livache

6. Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis

            Fang Cheng and Daniel M. Ratner

7. Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan-Protein Interactions

            Myung-Ryul Lee, Sungjin Park, and Injae Shin

8. Neoglycolipid (NGL)-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Non-Covalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses

            Yan Liu, Robert A. Childs, Angelina S. Palma, Maria A. Campanero-Rhodes, Mark S. Stoll, Wengang Chai, and Ten Feizi

9. Preparation of a Mannose-6-phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-mannose N-glycans and its Application in Ligand Identification of P-type Lectins

            Xuezheng Song, Jamie Heimburg-Molinaro, Nancy M. Dahms, David F. Smith, and Richard D. Cummings

10. Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

            Sahana K. Nagappayya and Nicola L. B. Pohl

11. General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides

            Yalong Zhang and Jeffrey C. Gildersleeve

12. Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays

            Oxana E. Galanina, Alexander A. Chinarev, Hadezha V. Shilova, Marina A. Sablina, and Nicolai V. Bovin

13. Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray

            Matthew J. Linman, Hai Yu, Xi Chen, Quan Cheng

14. Glycoarray by DNA Directed Immobilization

            Yann Chevolot, Jing Zhang, Albert Meyer, Sébastien Vidal, Jean-Pierre Praly, Jean-Jacques Vasseur, Eliane Souteyrand, and François Morvan

15. Fabrication of Carbohydrate Surfaces by Using Non Derivatised Oligosaccharides

            Jonathan Popplewell, Marcus Swan, Gavin Brown, and Bob Lauder

16.  Polysaccharide Microarrays. Application to the Identification of Heparan Sulfate Mimetics

            Justin Dheur, Nabil Dendane, Rémi Desmet, Fatima Dahmani, Gauthier Goormachtigh, Jérome Vicogne, Véronique Fafeur, Oleg Melnyk

17. Carbohydrate Antigen Microarrays

            Denong Wang

18. Probing Virus-Glycan Interactions Using Glycan Microarrays

            Jamie Heimburg-Molinaro, Mary Tappert, Xuezheng Song, Yi Lasanajak, Gillian Air, David F. Smith, and Richard D. Cummings

19. MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays

            Nicolas Laurent, Rose Haddoub, Josef Voglmeir, and Sabine L. Flitsch

20. Microarray Technology using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection

            Emanuela Lonardi, André M. Deelder, Manfred Wuhrer, and Crina I.A. Balog

21. Studying Modification of Aminoglycoside Antibiotics by Resistance-causing Enzymes via Microarray

            Matthew D. Disney

22. Microarray Method for the Rapid Detection of Glycosaminoglycan-Protein Interactions

            Claude J. Rogers, and Linda C. Hsieh-Wilson

23. Neoglycolipid-based ‘Designer’ Oligosaccharide Microarrays to Define b-glucan Ligands for Dectin-1

            Angelina S. Palma, Yibing Zhang, Robert A. Childs, Maria A. Campanero-Rhodes, Yan Liu, Ten Feizi, Wengang Chai

24. Measurement of Antibodies to Pneumococcal Polysaccharides with Luminex xMAP Microsphere-based Liquid Arrays

            Jerry W. Pickering and Harry R. Hill

25. Carbohydrate Microarrays in 96-well Polystyrene Microtiter Plates

            Jean-Phillipe Ebran, Nabil Dendane, and Oleg Melnyk

26. Photoimmobilization of Saccharides

            Gregory T. Carroll and Denong Wang

27. Microwave Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydrazide-Dendrimer Substrate

            Xichun Zhou, Jian Zhang, and Denong Wang

28. Combinatorial Glycoarray

            Simon Rinaldi, Kathryn M. Brennan, and Hugh J. Willison