Phage Display

A Practical Approach
Erscheinungsjahr 2004
ISBN: 978-0-19-963873-4
Verlag: Oxford University Press

Buch, Englisch, 360 Seiten, Print PDF, Format (B × H): 189 mm x 246 mm, Gewicht: 715 g

This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.

Autoren/Hrsg.

Clackson, Tim

Lowman, Henry B.

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tiergenetik, Reproduktion
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)

Inhaltsverzeichnis

- 1: Russel, Lowman and Clackson: Introduction to phage biology and phage display

- 2: Sidhu and Weiss: Constructing phage display libraries by oligonucleotide-directed mutagenesis

- 3: Miyazaki and Arnold: In vitro DNA recombination

- 4: Dennis and Lowman: Phage selection strategies for improved affinity and specificity of proteins and peptides

- 5: DeLano and Cunningham: Rapid screening of phage displayed protein binding affinities by phage ELISA

- 6: Cwirla et al: Identification of novel ligands for receptors using recombinant peptide libraries

- 7: Matthews and Ballinger: Substrate phage display

- 8: Tuna et al: Protease-based selection of stably folded proteins and protein domains from phage display libraries

- 9: Isalan and Choo: Phage display of zinc fingers and other nucleic acid-binding motifs

- 10: Hoffman et al: In vivo and ex vivo selections using phage-displayed libraries

- 11: Monaci and Cortese: Screening phage libraries with sera

- 12: Jespers and Fransen: Interaction cloning using cDNA libraries displayed on phage

- 13: Bradbury and Marks: Phage antibody libraries

- 14: Neilson and Marks: Affinity maturation of phage antibodies

Über Autor(innen)

Dr Tim Clackson
Vice President, Gene Therapy and Genomics
ARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA
[phone] ext. 258
[phone]
[e-mail]
Tim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years in a variety of research and research management positions. His group is combining chemistry, cell biology and protein engineering to develop techniques for regulating gene expression and other intracellular processes using small molecules. Dr Clackson's particular research interest is the use of protein engineering and chemical biology techniques to analyze and redesign the interfaces between molecules, especially small molecule-protein interfaces.

Dr Henry B. Lowman
Senior Scientist
Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA
[phone]
[phone]
[e-mail]
Henry B. Lowman, Ph.D. is a Senior Scientist and Associate Director of Antibody Engineering with joint appointments in Immunology and Protein Engineering, at Genentech, Inc., where he has been engaged in research on proteins and peptides for over thirteen years.