Rapid Cell Magnetisation Using Cationised Magnetoferritin

Softcover Nachdruck of the original 1. Auflage 2017
ISBN: 978-3-319-86838-7
Verlag: Springer International Publishing

Buch, Englisch, 166 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 2876 g

Reihe: Springer Theses

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently. 

Zielgruppe

Research

Autoren/Hrsg.

Correia Carreira, Sara

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Physik Elektromagnetismus Magnetismus
• Technische Wissenschaften Technik Allgemein Nanotechnologie
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Chemie Physikalische Chemie Molekulare Chemische Nanostrukturen

Inhaltsverzeichnis

Introduction.- Materials and Methods.- Characterising Magnetoferritin and Cationised Magnetoferritin.- Stem Cell Labelling with Cationised Magnetoferritin.- Toxicological Profiling of Cationised Magnetoferritin.- Escherichia coli labelling with Cationised Magnetoferritin.- Overall Conclusions and Future Opportunities.