De Francesco / Turksen | Wound Regeneration | Buch | 978-1-0716-5613-6 | www.sack.de

Buch, Englisch, 436 Seiten, Format (B × H): 178 mm x 254 mm

Reihe: Methods in Molecular Biology

De Francesco / Turksen

Wound Regeneration

Methods and Protocols
Erscheinungsjahr 2026
ISBN: 978-1-0716-5613-6
Verlag: Springer Us

Methods and Protocols

Buch, Englisch, 436 Seiten, Format (B × H): 178 mm x 254 mm

Reihe: Methods in Molecular Biology

ISBN: 978-1-0716-5613-6
Verlag: Springer Us


This detailed volume gathers a comprehensive collection of state-of-the-art experimental and clinical protocols focused on wound regeneration. The book reflects the multidisciplinary nature of regenerative medicine and includes standardized methodologies spanning in vitro assays, preclinical animal models, cellular and biologic therapies, extracellular vesicle isolation, biomaterial development, scaffold engineering, micrografting technologies, and translational clinical applications. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, serves not only as a practical laboratory and clinical resource but also as an inspiration to stimulate further innovation and interdisciplinary collaboration.

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Professional/practitioner

Weitere Infos & Material


Current Therapeutic Strategies and Emerging Technologies in Skin Wound Repair.- Wound Healing Assay Using Xenopus Embryonic Ectoderm.- In Vitro Assays for Keloid Fibroblast Activity and Wound Regeneration.- Wound Healing In Vitro Protocols for Space Biomedicine Insights.- Protocols for Immunohistochemistry and Immunofluorescence Studies of Wound Healing in Porcine Cornea and Skin.- Nitrogen Mustard-Induced Wound Model for Epithelial Tissue.- Inducible Systems of Genetically Engineered Mouse Models Provide Valuable Tools for Elucidating Gene Function During Wound Healing.- Preparation of Stromal Vascular Fraction (SVF) for Wound Regeneration: A Standardized Clinical Protocol.- Preparation of Bone Marrow Aspirate Concentrate (BMAC): A Standardized Clinical Protocol for Wound Regeneration.- Preparation of Platelet-Derived Biologics for Wound Regeneration: A Standardized Point-of-Care Protocol.- Preparation of Platelet Lysate (PL): A Standardized Protocol for Clinical and Research Applications.- In Vitro Assessment of Advanced Platelet-Rich Fibrin Plus (A-PRF+) by Wound Healing Assay in Equine Fibroblasts.- Preparation and Application of Equine Advanced Platelet-Rich Fibrin Plus (A-PRF+) on Traumatic Wounds.- Isolation of Adipose-Derived Exosomes for the Repair of Tissue Injury Associated with Cardiovascular Diseases.- Fibrin Gel Synthesis for MSC Seeding and Exosome Derivation.- Mechanical Disaggregation of Adipose Tissue Using Rigenera® Technology: A Protocol for Wound Healing Applications.- Regenerative and Biostimulant Injectables in Skin and Oral Wound Healing: Evidence from Experimental Models.- Protocol for Hair-Follicle-Associated Pluripotent (HAP)-Stem-Cell-Sheet Implantation to Heal Cutaneous Wounds in Mice.- Decellularized Dermal Extracellular Matrix for Wound Healing Research and Applications.- Clinical Application of Cryopreserved Total Skin Allografts from Living Donors for Regenerative Wound Care.- Preparation and Evaluation of Graphene-Based Biomaterials for Wound Healing.- Protocol for 3D Bioprinting of Alginate/Gelatin-Based and Co-Cultured Wound Healing Constructs.- Wound Regeneration Using Fish Skin Collagen Hydrogel.- Engineering and Analysis of Human Microvascular Networks in Microfluidic Devices: A Protocol Combining Live Imaging, Permeability Assays, and Machine Learning-Based Vascular Quantification.- Enhancement of Hair Transplant Efficiency by Micrograft Preconditioning in Scarred Scalp Tissue.- From Planimetry to Pathology: A Porcine Full-Thickness Burn Model to Evaluate Regenerative Skin Therapies.- Surgical Reconstructive Strategies for Burn Wounds: From Autografting to Vascularized Composite Allotransplantation.- Clinical-to-Laboratory Workflow for Standardized Wound Tissue Collection and Pre-Analytic Processing.



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