Buch, Englisch, 286 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 561 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 286 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 561 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-6125-2
Verlag: Humana
Provides detailed protocols for a wide variety of SPR applications Includes comprehensive background information on SPR techniques for better experimental design Examines a variety of coupling techniques on different sensor surfaces Highlights background issues involved in SPR assays to avoid common errors frequently found in SPR literature Presents protocols in clear step by step procedures including the basics of kinetic analysis Focuses on a wide range of applications for membrane-bound protein interactions, including G protein-coupled receptors Chapters on the combination of SPR with mass spectrometry (SPR-MS) for quantification and identification of proteins Written for users of commercially available SPR equipment Covers the two most common sources of deviation from ideal kinetics: mass transport and surface heterogeneity Includes supplementary material: sn.pub/extras
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Surface Plasmon Resonance: A General Introduction.- The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing.- Amine Coupling Through EDC/NHS: A Practical Approach.- High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies.- A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance.- Affinity Constants for Small Molecules from SPR Competition Experiments.- Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules.- High-Throughput Kinase Assay Based on Surface Plasmon Resonance.- SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads.- Carbohydrate–Lectin Interactions Assayed by SPR.- DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films.- Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors.- Capture of Intact Liposomes on Biacore Sensor Chips for Protein–Membrane Interaction Studies.- Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides.- Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid ? Proteins (A?) and Lipid Membranes.- Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies.- Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling.- Integration of SPR Biosensors with Mass Spectrometry (SPR-MS).- SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry.
