Buch, Englisch, Band 284, 327 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 284, 327 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-58829-245-2
Verlag: Humana Press
Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Making Protein Immunoprecipitates
Elaine A. Elion and Yunmei Wang
Signal Transduction Inhibitors in Cellular Function
Maofu Fu, Chenguang Wang, Xueping Zhang, and Richard G. Pestell
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, and Natalie G. Ahn
A High-Throughput Mammalian Cell-Based Transient Transfection Assay
Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski
Determining Protein Half-Lives
Pengbo Zhou
Assaying Protein Kinase Activity
Jan Brábek and Steven K. Hanks
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
Francesca Zappacosta, Michael J. Huddleston, and Roland S. Annan
Studies of Calmodulin-Dependent Regulation
Paul C. Brandt and Thomas C. Vanaman
Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, and Emery H. Bresnick
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
Laurent Kuras
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
Douglas A. Andres
Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping
Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart
Techniques in Protein Methylation
Jaeho Lee, Donghang Cheng, and Mark T. Bedford
Assaying Lipid Phosphate Phosphatase Activities
Gil-Soo Han and George M. Carman
Assaying Phosphoinositide Phosphatases
Gregory S. Taylor and Jack E. Dixon
Assaying Phospholipase A2 Activity
Christina C. Leslie and Michael H. Gelb
Measurement and Immunofluorescence of Cellular Phosphoinositides
Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, and Daryll B. DeWald
Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, and Manuela Zaccolo
In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, and Albrecht G. von Arnim
Revealing Protein Dynamics by Photobleaching Techniques
Frank van Drogen and Matthias Peter
Assaying Cytochrome c Translocation During Apoptosis
Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, and Joseph A. Trapani
Index
