Tools and Techniques in Biomolecular Science

Erscheinungsjahr 2013
ISBN: 978-0-19-969556-0
Verlag: OUP Oxford

Buch, Englisch, 528 Seiten, Format (B × H): 195 mm x 264 mm, Gewicht: 1124 g

Tools and Techniques in the Biomolecular Sciences reviews the theoretical concepts and experimental details underpinning the broad range of modern technologies that are currently being used to advance our understanding of the biomolecular sciences.

Each chapter focuses on the theoretical principles of a given technology, and its applications and limitations, before using worked examples to illustrate the nature of the data a particular technique generates.

Fostering active engagement with the techniques presented, each chapter uses worked examples and problems to encourage the reader to critically appraise scientific data and select the most appropriate methods when designing an experimental strategy to undertake their own research.

Its straightforward style and clear explanations make Tools and Techniques in the Biomolecular Sciences the ideal resource for any student or early-stage researcher who is looking to develop a firm understanding of the range of experimental tools at their disposal.

Online Resource Centre:

For registered adopters:

Figures from the book in electronic format

For students:

Additional questions for students, with model answers

Journal Clubs - selected papers and discussion papers related to topics featured in the book

Zielgruppe

Undergraduates and beginning graduates studying or carrying out biomolecular techniques as part of a bioscience or biomedical science degree programme or doctoral training programme.

Autoren/Hrsg.

Divan, Aysha

Royds, Janice

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

- 1: Gene Cloning Essentials David Hornby and Qaiser I Sheikh

- 2: PCR Martin Evison

- 3: DNA Mutagenesis Sarah E. Deacon and Michael J. McPherson

- 4: DNA Sequencing Tania Slatter and Alison Fitches

- 5: Measuring DNA/Protein Interactions Luis Acevedo, Ana Sanz, Paul Labhart and Mary Anne Jelinek

- 6: RNA Interference Technology Gavin Batman, Thomas Walker and Ian Hampson

- 7: Recombinant Protein Expression Michael Harrison and Michael J. McPherson

- 8: Protein Purification Iain Manfield

- 9: Antibodies as Research Tools Gavin Allsop and John Colyer

- 10: Measuring Protein-Protein Interactions: Qualitative Approaches Johanna M. Avis and Sanjay Nilapwar

- 11: Measuring Protein-Protein Interactions: Quantitative Approaches Johanna M. Avis and Sanjay Nilapwar

- 12: Structural Analysis of Proteins: X-ray Crystallography, NMR, AFM, and CD spectroscopy Thomas Edwards, Arwen Pearson, Gary Thompson, Arnout Kalverda, Oliver Farrance, David Brockwell and Gareth Morgan

- 13: Mass Spectrometry James R. Ault and Alison E. Ashcroft

- 14: Proteomic Analysis Richard Unwin

- 15: Culturing Mammalian Cells Geoffrey J Pilkington, Suzanne M. Birks and Laura K. Donovan

- 16: Flow Cytometry John Lawry

- 17: Bioimaging: Light and Electron Microscopy Gareth Howell and Kyle Dent

- 18: Histopathology in Biomolecular Research Noelyn Hung and Tania Slatter

- 19: Mouse Models in Bioscience Research Brian Corbett and Jeannie Chin

- 20: Mathematical Models in Biomolecular Sciences Josie Athens

Über Autor(innen)

Dr Aysha Divan is a lecturer in the Faculty of Biological Sciences at the University of Leeds, where her laboratory-based research includes tumour biology, and her pedagogical research examines postgraduate-level communication and technical skills training in the biosciences.

Dr Janice Royds is a lecturer in the Department of Pathology at the University of Otago, New Zealand. Her broad ranging biomedical research interests relate to cancer biology, while her extensive teaching experience at both undergraduate and postgraduate level include pathology, pharmacology, and the molecular biology of cancer.