Quantitative Methods in Neuroscience

A Neuroanatomical Approach
Erscheinungsjahr 2004
ISBN: 978-0-19-850528-0
Verlag: Oxford University Press

Buch, Englisch, 350 Seiten, Format (B × H): 164 mm x 237 mm, Gewicht: 690 g

Stereology is a valuable tool for neuroscientists, allowing them to obtain 3-Dimensional information from 2-Dimensional measurements made on appropriately sampled sections (usually obtained from histological sections or MRI/CT/PET scans). This 3-D information is invaluable in correlating structural/functional relationships in the pursuit of far greater understanding of the function of the central nervous system. However, in carrying out such measurements, often based on limited data sets, there is a risk of experimenter bias. An important feature of modern design based stereology is to be aware of potential sources of bias and eliminate them during the data collection. With many of the major neuroscience journals now insisting that quantitative data be presented, there is a greater need than ever for neuroscientists to understand the theory and practice behind quantitative methods, such as those offered by stereology.

Quantitative Methods in Neuroscience is a cookbook of stereological methods written especially for neuroscientists. It provides clear and accessible advice about when and when not to use stereology. Throughout the book, the emphasis is on practical guidance, rather than discussions and formulae. Written by leading scientists in the field of stereology, with a Foreword by D.C. Sterio, the book will be a valuable introduction to these methods for neuroscientists, and all those involved in development of new drug programmes.

Autoren/Hrsg.

Evans, Stephen M

Janson, Ann Marie

Nyengaard, Jens Randel

Fachgebiete

• Interdisziplinäres Wissenschaften Wissenschaften Interdisziplinär Neurowissenschaften, Kognitionswissenschaft
• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren
• Naturwissenschaften Biowissenschaften Biowissenschaften Neurobiologie, Verhaltensbiologie

Inhaltsverzeichnis

- 1: Stephen M. Evans and Jens Randel Nyengaard: Introduction

- 2: Luis Cruz-Orive, A.M. Insausti, R. Insausti and D. Crespo: A case study from neuroscience involving stereology and multivariate analysis

- Section 1: Number

- 3: Stephen M. Evans and Jens Randel Nyengaard: Introduction

- 4: Daniel A. Peterson: The use of fluorescent probes in cell counting procedures

- 5: Bente Finsen, R. Gregersen, E. Lehrmann, S. Lovmand and M.J. West: Counting in situ hybridized neurons

- 6: Jens Randel Nyengaard, K-A. Dorph-Petersen, Y. Tang: Number in electron microscopy: estimation of total number of synapses in the main regions of human neocortex

- 7: Annemette Lokkegaard: The number of microvessels estimated by an unbiased stereological method applied in a brain region

- Section 2: Volume

- 8: Stephen M. Evans and Jens Randel Nyengaard: Introduction

- 9: Trine Tandrup: The nucleator and the planar and optical rotators applied in rat dorsal root ganglia

- 10: Karl-Anton Dorph-Petersen, Raben Rosenberg and Jens Randel Nyengaard: Estimation of number and volume of immunohistochemically stained neurons in complex brain regions

- Section 3: Length

- 11: Stephen M. Evans and Jens Randel Nyengaard: Introduction

- 12: Yong Tang and Jens Randel Nyengaard: Length estimation of nerve fibers in human white matter using isotropic uniformly random sections

- 13: Jytte Overgaard Larsen, M. von Euler and Ann Marie Janson: Virtual test systems for estimation of orientation- dependent parameters in thick, arbitrary oriented sections exemplified by length quantification of regenerating axons in spinal cord lesions using isotropic, virtual planes

- Section 4: Second order stereology

- 14: Stephen M. Evans: Spatial distribution

- Section 5: Cell Culture

- 15: Hannsjorg Schroder, Jens Rendel Nyengaard, W. Selberis, E. Lain, A. Keller, C. Kohler and B. Witter: Unbiased morphometrical techniques for the quantitative assessment of cells in primary dissociation cultures