Expansion Microscopy for Cell Biology

Erscheinungsjahr 2021
ISBN: 978-0-12-820807-6
Verlag: William Andrew Publishing

Buch, Englisch, 356 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 950 g

Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.

Zielgruppe

<p>Researchers in the area of Cell biology, Plant biology, Neurobiology as well as other scientific areas.</p>

Autoren/Hrsg.

Weitere Mitwirkende

Guichard, Paul

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie

Inhaltsverzeichnis

1. Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion
Paul Tilberg
2. Ultrastructure Expansion Microscopy (U-ExM)
Davide Gambarotto, Virginie Hamel and Paul Guichard
3. Expansion STED microscopy (ExSTED)
Helge Ewers
4. Simple multi-color super-resolution by X10 microscopy
Sven Truckenbrodt
5. Expansion microscopy imaging of various neuronal structures
Jae-Byum Chang
6. Mapping the neuronal cytoskeleton using expansion microscopy
Lukas Kapitein
7. Mechanical expansion microscopy
Bo Wang
8. Enhanced expansion microscopy to measure nanoscale structural and biochemical remodelling in single cells
Izzy Jayasinghe
9. Application of Expansion Microscopy on Developing Arabidopsis Seeds
Michael D. Nodine
10. A protocol to expand plant nuclei
Veit Schubert
11. Expansion microscopy of the mitotic spindle
Ivana Ponjavic
12. Expansion microscopy at the nanoscale. The nuclear pore complex as fiducial landmark
Paolo Bianchini
13. Post-labeling expansion microscopy, a promise to go beyond super-resolution limitations
Paul Guichard and Virginie Hamel
14. Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures
Markus Sauer
15. Expansion Microscopy on Drosophila Spermatocyte Centrioles
Alan Wainman

Über Autor(innen)

Guichard, Paul
Paul Guichard is an expert on the structure of the centriole. From 2007 to 2010, he did his PhD at the Institut Curie (France) under the co-direction of Drs. Tassin and Marco, on the study of the human procentriole assembly using cryo-tomography. From 2011 to 2015, he did his post-doctoral work in the laboratory of Prof. Gönczy at EPFL (Lausanne, Switzerland) where he studied the structure of the centriolar cartwheel by combining cryo-tomography, in vitro assemblies and super-resolution microscopy. In 2015, together with Dr. Hamel, they created the Centriole Architecture Laboratory at the University of Geneva (Switzerland), aiming at revealing the molecular architecture and function of the centriole.