Chikungunya Virus

Methods and Protocols
Erscheinungsjahr 2026
ISBN: 978-1-0716-5495-8
Verlag: Springer Us

Buch, Englisch, 526 Seiten, Format (B × H): 178 mm x 254 mm

Reihe: Methods in Molecular Biology

This volume presents laboratory protocols to study different aspects of CHIKV biology, virus RNA and protein functions, virus-host interactions, pathogenesis, immune responses, diagnostic techniques, structural biology, vector competence and surveillance, antiviral strategies and treatment. Written for the highly successful   series, chapters include the kind of detailed implementation advice needed to ensure success in the lab.

 Cutting-edge and authoritative, provides specific lab procedures, techniques, and applications to prepare for future outbreaks of arboviral diseases.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Filomatori, Claudia

Castro, Eliana

Fachgebiete

• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung

Inhaltsverzeichnis

Overview of chikungunya virus epidemiology, biology, and pathogenesis.- Naked-eye visual loop-mediated isothermal amplification method for the rapid detection of chikungunya virus.- RT-RPA assisted CRISPR/Cas12a based Isothermal Detection of Chikungunya virus.- Quantification of Chikungunya Virus RNA by Real-Time RT-PCR Assay.- Determination of Chikungunya Virus Infectious Titer Using Plaque Assays.- Automated Counting of Chikungunya Virus–Formed Plaques.- Production of Viral Particles from a Chikungunya Virus Infectious Clone.- Molecular Cloning and Reverse Genetics.- A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids.- Viral Protein Expression and Purification.- PAGE-based measurements of nsP2 protease activity.- Assessing Chikungunya Virus Protease Activity and Pharmacological Inhibition Using the acc-CHIK15-dnp Fluorogenic Peptide Substrate.- Epitope tagging and coimmunoprecipitation to identify viral protein interactors.- Plaque reduction neutralization test against chikungunya virus using liver homogenate.- Screening of Antiviral Agents against CHIKV using reporter virus.- In silico drug design of chikungunya antivirals: using consensus methods for virtual screening.- Surface Plasmon Resonance-Based Analysis of Ligand Binding Kinetics in Chikungunya Virus Drug Discovery.- Analysis of cell populations from arbovirus infected tissues by flow cytometry.- Chikungunya virus infection in paraffin-embedded tissue: analysis of histological alterations and viral detection by immunohistochemistry.- Collection and processing of adult mosquitoes (Diptera: Culicidae) for molecular studies of arbovirus infection.- Assessing the Vector Competence for Chikungunya Virus in Mosquitoes.- Global Genomic Surveillance.- Nanopore Sequencing for Chikungunya Virus: Principles and Application.- Workflow for Long-Read Amplicon Sequencing of Chikungunya Virus Using Oxford Nanopore Technology.- Optimized Amplicon Strategy for Long-Read Sequencing of the Chikungunya Virus Genome.- Protocol for detecting and sequencing chikungunya virus from field-collected mosquitoes.