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Gibbins / Mahaut-Smith Platelets and Megakaryocytes



Volume 2: Perspectives and Techniques

1. Auflage. Softcover version of original hardcover Auflage 2004, Band: 273, 504 Seiten, Kartoniert, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 767 g Reihe: Methods in Molecular Biology
ISBN: 978-1-61737-305-3
Verlag: Springer, Berlin


Gibbins / Mahaut-Smith Platelets and Megakaryocytes

12 The average human body has on the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in Western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well-established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.

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Weitere Infos & Material


Part I. Perspectives
Signaling Receptors on Platelets and Megakaryocytes
Donna Woulfe, Jing Yang, Nicolas Prevost, Peter O'Brien, Ryan Fortna, Massimiliano Tognolini, Hong Jiang, Jie Wu, and Lawrence F. Brass
Manipulation of Gene Expression in Megakaryocytes
Nikla R. Emambokus, George J. Murphy, and Jonathan Frampton
In Vitro Changes of Platelet Parameters: Lessons From Blood Banking
José Rivera, María L. Lozano, and Vicente Vicente
Pharmacological Approaches to Studying Platelet Function: An Overview
Susanna M. O. Hourani
Inhibitors of Cellular Signaling Targets: Designs and Limitations
Chris J. Vlahos and Louis F. Stancato Part II Signaling Techniques. Ligand-Binding Assays for Collagen
Stephanie M. Jung and Masaaki Moroi
Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines
Pierre Savi and Jean-Marc Herbert
Ligand-Binding Assays: Fibrinogen
Dermot Cox
Techniques for the Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting
Jonathan M. Gibbins
Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation
Jonathan M. Gibbins
Protein Tyrosine Phosphatases
Matthew L. Jones and Alastair W. Poole
The Study of Serine-Threonine Kinases
Gertie Gorter and Jan Willem Akkerman
Phosphoinositides: Lipid Kinases and Phosphatases
Bernard Payrastre
Isolation and Analysis of Platelet Lipid Rafts
Corie N. Shrimpton, Karine Gousset, Fern Tablin, and José A. López
Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures
Philippe Ohlmann, Béatrice Hechler, Jean-Pierre Cazenave, and Christian Gachet
Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes
Michael J. Mason and Martyn P.Mahaut-Smith
Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte
Martyn P. Mahaut-Smith
Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets
Khalid M. Naseem, Rocio Riba, and Max Troxler
Nitric Oxide Signaling in Platelets
Sylvia Y. Low and K. Richard Bruckdorfer
Snake Venom Toxins Affecting Platelet Function
Robert K. Andrews, Elizabeth E. Gardiner, and Michael C. Berndt
Peptide Synthesis in the Study of Collagen-Platelet Interactions
C. Graham Knight, Catherine M. Onley, and Richard W. Farndale
Platelet Permeabilization
Robert Flaumenhaft
Part III. Molecular and Post-Genomic Techniques
Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells
Meenakshi Gaur, George J. Murphy, Jonathan Frampton, and Andrew D. Leavitt
Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes
Hava Avraham, Shalom Avraham, and Radoslaw Zagozdzon
GFP Fusion Proteins to Study Signaling in Live Cells
Simon A. Walker, Gyles E. Cozier, and Peter J. Cullen
Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics
Katrin Marcus and Helmut E. Meyer
Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes
Benjamin Z. S. Paul, Jiango Jin, and Satya P. Kunapuli
Platelet Receptor Structures and Polymorphisms
Thomas J. Kunicki, Steven Head, and Daniel R. Salomon
Gene Array Technology and the Study of Platelets and Megakaryocytes
Lloyd T. Lam and Emery H. Bresnick
Index


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