Buch, Englisch, 274 Seiten, Print PDF, Format (B × H): 156 mm x 234 mm, Gewicht: 424 g
A Practical Approach Volume 2: Expression Systems
Buch, Englisch, 274 Seiten, Print PDF, Format (B × H): 156 mm x 234 mm, Gewicht: 424 g
ISBN: 978-0-19-963478-1
Verlag: OUP Oxford
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
Autoren/Hrsg.
Fachgebiete
- Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
- Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
- Naturwissenschaften Biowissenschaften Zellbiologie
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
- Naturwissenschaften Biowissenschaften Proteinforschung
Weitere Infos & Material
- 1.: Screening expression libraries with antibody and protein probes
- 2.: Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
- 3.: Purification of over-produced proteins from E.coli cells
- 4.: Production of monoclonal antibodies against proteins expressed in E.coli
- 5.: Expression of cloned genes in yeast
- 6.: Intraction trap cloning with yeast
- 7.: The baculovirus expression system
