Isolation and Purification of Proteins

1. Auflage 2003
ISBN: 978-0-8247-0726-2
Verlag: CRC Press

Buch, Englisch, 676 Seiten, Format (B × H): 161 mm x 239 mm, Gewicht: 1016 g

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Zielgruppe

Professional Practice & Development

Autoren/Hrsg.

Hatti-Kaul, Rajni

Mattiasson, Bo

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie
• Naturwissenschaften Chemie Organische Chemie Polymerchemie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Verfahrenstechnik, Chemieingenieurwesen

Inhaltsverzeichnis

Resolution technique for protein separation, selectivity in affinity chromatography, genetic approaches to facilitate purification, solid-liquid separation, membranes for protein isolation and purification, precipitation of proteins - non-specific and specific, protein crystallization for large-scale bioseparation, extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, fast chromatography of proteins, novel approaches to the chromatography of proteins, scale up and commercial production of recombinant proteins, process monitoring in downstream processing, protein formulation, regulatory requirements, cleaning and sanitization issues in protein purification.

Über Autor(innen)

Rajni Hatti-Kaul, Bo Mattiasson