Buch, Englisch, Band 1928, 495 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1143 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1928, 495 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1143 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-9026-9
Verlag: Springer
This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories.
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Weitere Infos & Material
1 Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction
Daniel R. Crooks, Teresa W-M. Fan, and W. Marston Linehan
2 Imaging Cancer Metabolism with Positron Emission Tomography (PET)
Timothy H. Witney and David Y. Lewis
3 Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells
Debanti Sengupta and Guillem Pratx
4 Use of C3N1-Serine or C5N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics
Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D. K. Maddocks
5 Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)
Sarah Creed and Matthew McKenzie
6 Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells
David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, and Carmen Chak-Lui Wong
7 Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment
Ioannis S. Pateras and Tomer Cooks
8 Quantitation of Macropinocytosis in Cancer Cells
Koen M.O. Galenkamp, Basheer Alas, and Cosimo Commisso
9 Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation
Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, and Alessandro Carrer
10 Methods to Measure Autophagy in Cancer Metabolism
Natalia Von Muhlinen
11 Lipidomic Analysis of Cancer Cell and Tumor Tissue
Sk Ramiz Islam and Soumen Kanti Manna
12 Mass Spectrometry-based Profiling of Metabolites in Human Biofluids
Tanushree Chakraborty and Soumen Kanti Manna
13 Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar MetabolitesJodi Rattner, Farshad Farshidfar, and Oliver F. Bathe
14 Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy
Beata Mickiewicz, M. Eric Hyndman and Hans J. Vogel
15 Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric ImagingShibdas Banerjee and Soumen Kanti Manna
16 Compositional Analysis of the Human Microbiome in Cancer Research
Elisa Morales, Jun Chen, and K. Leigh Greathouse
17 Assessing Metabolic Dysregulation in Muscle During Cachexia
Myriam Y Hsu, Paolo E Porporato, and Elisabeth Wyart
18 Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells
Jing Zhang and Qing Zhang
19 Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging
Thomas S. Blacker, Michael D.E. Sewell, Gyorgy Szabadkai, and Michael R. Duchen
20 Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods
Amanda J. Pearson and Elyssia S. Gallagher
21 Hyperpolarized MRI for Studying Tumor Metabolism
Mikko I. Kettunen22 Overview of Glutamine-Dependency and Metabolic Rescue Protocols
Shuo Qie, Dan He, and Nianli Sang
23 Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype
Andy Patt, Jalal Siddiqui, Bofei Zhang, and Ewy Mathé
24 Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways
Robert B. Bentham, Kevin Bryson, and Gyorgy Szabadkai
25 Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism
Lake-Ee Quek and Nigel Turner
