Capillary Electrophoresis: Principles and Practice

Softcover Nachdruck of the original 1. Auflage 1993
ISBN: 978-3-642-78060-8
Verlag: Springer

Buch, Englisch, 375 Seiten, Paperback, Format (B × H): 155 mm x 235 mm, Gewicht: 587 g

Reihe: Springer Lab Manuals

Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench.An addendum includes tables on the preparation of buffers and recommended further reading.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hoffstetter-Kuhn, Sabrina

Kuhn, Reinhard

Inhaltsverzeichnis

1 Introduction.- 1.1 Safety Considerations.- 1.2 History.- 1.3 Nomenclature.- 2 Basic Principles.- 2.1 Basic Electrophoretic Separation Modes.- 2.2 Set-up for Capillary Electrophoresis.- 2.3 Theory of Electrophoretic Migration.- 2.4 Determination of Effective Mobilitiy.- 2.5 Electroosmosis.- 2.6 Performance Criteria.- 3 Factors Influencing Performance.- 3.1 Fundamental Dispersive Effects.- 3.2 Operational Parameters.- 3.3 Electrolyte System.- 4 Instrumentation.- 4.1 Injection.- 4.2 Detection.- 4.3 Capillary Column.- 4.4 Sample Collection.- 4.5 Commercial Instruments.- 5 Techniques.- 5.1 Capillary Zone Electrophoresis.- 5.2 Capillary Gel Electrophoresis.- 5.3 Micellar Electrokinetic Chromatography.- 5.4 Capillary Isotachophoresis.- 5.5 Capillary Isoelectric Focusing.- 5.6 Electrochromatography.- 5.7 Hyphenated Techniques.- 5.8 Special Techniques.- 6 Qualitative and Quantitative Analysis.- 6.1 General Aspects.- 6.2 Influence of Injection.- 6.3 Method Validation.- 7 Applications.- 7.1 Small Ions.- 7.2 Sulphonates and Alkylsulphates.- 7.3 Drugs and Natural Products.- 7.4 Neutral Substances.- 7.5 Herbicides.- 7.6 Amino Acids, Peptides and Proteins.- 7.7 Carbohydrates and Their Derivatives.- 7.8 Nucleotides, Oligonucleotides and Nucleic Acids.- 7.9 Chiral Molecules.- 7.10 Complex Samples.- 8 Appendix 331.- 8.1 Buffer Tables.- 8.2 Derivatization Procedures.- 8.3 Glossary.- 8.4 Manufacturers’ Directory.- 8.5 Further Recommended Reading.- References.