Phage Display

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7446-7
Verlag: Springer

Buch, Englisch, Band 1701, 562 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 1266 g

Reihe: Methods in Molecular Biology

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.
This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lim, Theam Soon

Hust, Michael

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie

Inhaltsverzeichnis

Construction of Human Immune and Naïve scFv Libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of Synthetic Antibody Phage Display Libraries.- Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes.- Construction of Macaque Immune Libraries.- Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format.- Construction of Rabbit Immune Antibody Libraries.- Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries.- Construction of High-Quality Camel Immune Antibody Libraries.- Construction of Chicken Antibody Libraries.- Construction and Selection of Affilin Phage Display Libraries.- Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies.- Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach.- Parallelized Antibody Selection in Microtiter Plates.- Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection.- Phage Display and Selections on Cells.- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.- Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies.- Screening Phage Display Antibody Libraries using Protein Arrays.- Antibody Selection on FFPE Tissue Slides.- Antibody Affinity and Stability Maturation by Error Prone PCR.- Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data.- Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction.- High-Throughput IgG Reformatting and Expressions.- Monitoring Phage Biopanning by Next-Generation Sequencing.- ORFeome Phage Display.- Epitope Mapping by Phage Display.- Metasecretome Phage Display.- Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages.