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Idris Bone Research Protocols



Methods and Protocols

3rd Auflage 2019, Band: 1914, 670 Seiten, Gebunden, Book, Format (B × H): 180 mm x 258 mm, Gewicht: 1452 g Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8996-6
Verlag: Springer, Berlin


Idris Bone Research Protocols

The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.

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Weitere Infos & Material


Part I: In Vitro Culture of Bone Cells1. Isolation and Generation of OsteoclastsNadia Rucci, Alberta Zallone, and Anna Teti 2. Isolation and Generation of OsteoblastsInês P Perpétuo, Lucie E Bourne, and Isabel R Orriss  3. Isolation, Purification, Generation, and Culture of Osteocytes Jonathan H Gooi, Ling Yeong Chia, Christina Vrahnas, and Natalie A Sims 4. Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In VivoJanos Kanczler,  Rahul S. Tare, Peter D. Mitchell, Patrick Stumpf, Timothy J. Noble, Cameron Black, and  Richard O.C. Oreffo 5. 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction StudiesSilvia Marino, Ryan T. Bishop, Daniëlle De Ridder, Jesus Delgado-Calle, and Michaela R Reagan 6. Using Cell and Organ Culture Models to Analyse Responses of Bone Cells to Mechanical Stimulation Soraia P Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon Cf Rawlinson, and Andrew A Pitsillides Part II: Ex Vivo Culture of Bone Cells7. The ex vivo Organ Culture of BoneKatherine A. Staines, Genevieve Brown, and Colin Farquharson 8. Ex-Vivo Model Systems of Cancer-bone Cell InteractionsErdjan Salih 9. Mechanical Properties of Bone ex vivoSimon R. Goodyear and Richard M. Aspden Part III: Ex Vivo Culture of Bone Cells10. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation Silvia Marino and Aymen I. Idris  11. Analysis of Transcriptional Regulation in Bone Cells Huilin Jin, Antonia Sophocleous, Asim Azfer, and Stuart H Ralston 12. Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ HybridizationBrice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, and Francois Lamoureux Part IV: In Vivo Models of Bone 13. Ovariectomy/Orchiectomy in Rodents Antonia Sophocleous and Aymen I. Idris 14. In Vivo Models of Rheumatoid ArthritisBettina Grötsch, Aline Bozec and Georg Schett   15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus Antonia Sophocleous and Carmen Huesa 16. Models of Prostate Cancer Bone MetastasisSun Hee Park, Matthew Robert Eber, andYusuke Shiozawa  17. Animal Models of Breast Cancer Bone MetastasisClaudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell 18. Murine Models of Bone Sarcomas Camille Jacques, Nathalie Renema, Benjamin Ory,Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann 19. Mouse Models of Melanoma Bone MetastasisÖzge Uluçkan 20. Animal Models of Multiple MyelomaSam W.Z. Olechnowicz and Claire M. Edwards 21. The Calvarial Injection Assay Robert J. van 't Hof 22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides  23. Animal Models for the Study of Bone Derived PainAusten L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah  Part V: Microscopical and Imaging Techniques24. Histomorphometry in RodentsReinhold G. Erben and Martin Glösmann 25. Immunostaining of Skeletal TissuesAnke J. Roelofs and Cosimo De Bari 26. Techniques for the Study of Apoptosis in BoneVal Mann and Brendon Noble 27. Live Cell Imaging of Bone Cell and Organ CulturesSarah L. Dallas, Patricia A. Veno, and LeAnn M. Tiede-Lewis  28. Analysis of Bone Architecture in Rodents using micro Computed Tomography Robert J. van 't Hof  and Enrico Dall'Ara  29. Bone Measurements by Peripheral Quantitative Computed Tomography in RodentsJürg A. Gasser and Johannes Willnecker 30. Quantitative X-ray Imaging of Mouse Bone by FaxitronNatalie C Butterfield, John G Logan, Julian Waung, Graham R Williams, and JH Duncan Bassett 31. Scanning Electron Microscopy of BoneAlan Boyde 32. Transmission Electron Microscopy of BoneVincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, and Wouter Beertsen 33. Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy Gloria Allocca and Ning Wang 34. Fourier Transform Infrared Imaging of BoneEleftherios P. Paschalis 35. Raman Microscopy and BoneSimon R. Goodyear and Richard M. Aspden


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