The two-volume set IFIP AICT 363 and 364 constitutes the refereed proceedings of the 12th International Conference on Engineering Applications of Neural Networks, EANN 2011, and the 7th IFIP WG 12.5 International Conference, AIAI 2011, held jointly in Corfu, Greece, in September 2011. The 52 revised full papers and 28 revised short papers presented together with 31 workshop papers were carefully reviewed and selected from 150 submissions. The first volume includes the papers that were accepted for presentation at the EANN 2011 conference. They are organized in topical sections on computer vision and robotics, self organizing maps, classification/pattern recognition, financial and management applications of AI, fuzzy systems, support vector machines, learning and novel algorithms, reinforcement and radial basis function ANN, machine learning, evolutionary genetic algorithms optimization, Web applications of ANN, spiking ANN, feature extraction minimization, medical applications of AI, environmental and earth applications of AI, multi layer ANN, and bioinformatics. The volume also contains the accepted papers from the Workshop on Applications of Soft Computing to Telecommunication (ASCOTE 2011), the Workshop on Computational Intelligence Applications in Bioinformatics (CIAB 2011), and the Second Workshop on Informatics and Intelligent Systems Applications for Quality of Life Information Services (ISQLIS 2011).