Immuno-oncology and Immunotherapy Part E

Erscheinungsjahr 2025
ISBN: 978-0-443-29612-3
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.

Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.

Autoren/Hrsg.

Weitere Mitwirkende

Charpentier, Maud

Bravo-San Pedro, Jose Manuel

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie

Inhaltsverzeichnis

1. Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation
Douglas McNeel
2. Cancer cyro-immunotherapy: Models and methods
George Prendergast
3. Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology
Ileana Mauldin and Leon Zheng
4. In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes
Mario Tarantini, Anna Lisa GIULIANI, Valentina Vultaggio-Poma, Simonetta Falzoni, Elena Adinolfi and Francesco Di Virgilio
5. Analysis of the tumor microenvironment using imaging mass cytometry data
Zlatko Trajanoski and Zuzana Loncova
6. Saphenous vein blood collection for different immune analyses of living mice
Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla and Kassandra Ofelia Rodriguez-Aguillon
7. Use of mouse KO models to validate the specificity of monoclonal antibodies
George Prendergast, Lisa D. Laury-Kleintop, Summer Sedano and Kaylend Manley
8. Imaging mass cytometry to analyze the immune TME
Louis M. Weiner
9. Autoimmune arthritis: Transgenic mouse models and methods
Laura Mandik-Nayak, Lauren Merlo and Weidan Peng
10. Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments
Maha Ayyoub, Clara Maria SCARLATA, Virginie Feliu and Christel Devaud
11. Generation and functional evaluation of bispecific antibodies
Luis Alvarez-Vallina, Antonio Tapia Galisteo, Anaïs Jiménez-Reinoso, Rodrigo Lázaro Gorines and Belén Blanco
12. Isolation and phenotype analysis of intraepithelial lymphocytes from the mouse colon
Laura Bracci, Alessia Fiore, Michela Tozzi, Paola Rinzo, Daniele Macchia, Massimo Spada and Alessia Fabbri
13. Analysis of single cell TCR repertoires and gene expression from multi-modal scRNA-seq data
Dietmar Rieder, Christina Plattner and Gregor Sturm
14. Analysis of the tumor immune microenvironment of brain tumors by flow-cytometry
Claire I. Vanpouille-Box
15. Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time-lapse microscopy.
Fabrizio Mattei, Giovanna Schiavoni, Caterina Antonucci, Adriana Rosa Gambardella and Valentina Tirelli
16. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang

Über Autor(innen)

Bravo-San Pedro, Jose Manuel
Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).

Charpentier, Maud
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.