Buch, Englisch, Band 1670, 285 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 569 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1670, 285 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 569 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8444-2
Verlag: Springer
This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
1. Assessing Metabolic Flux in Plants with Radiorespirometry
Nicholas J. Kruger, Shyam K. Masakapalli, and R. George Ratcliffe
2. Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory FluxesToshihiro Obata, Laise Rosado-Souza, and Alisdair R. Fernie
3. A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution
Hardy Rolletschek and Gregor Liebsch
4. VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots
Aprajita Kumari and Kapuganti Jagadis Gupta
5. MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of Respiration
Peter Keil, Gregor Liebsch, Ljudmilla Borisjuk, and Hardy Rolletschek
6. Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds using Micro Sensor
Sonika Pandey, Aprajita Kumari, Bharadwaj C, and Kapuganti Jagadis Gupta
7. Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringe Infection
Pradeep Kumar Pathak and Kapuganti Jagadis Gupta
8. Measurement of Oxygen Status in Arabidopsis Leaves Undergoing Hypersensitive Response during Pseudomonas Infection
Aprajita Kumari, Gail Preston, and Kapuganti Jagadis Gupta
9. Isolation of Physiologically Active and Intact Mitochondria from Chickpea
Sonika Pandey, Aprajita Kumari, and Kapuganti Jagadis Gupta
10. Isolation and Structural Studies of Mitochondria from Pea Roots
Abhaypratap Vishwakarma and Kapuganti Jagadis Gupta
11. Mitochondrial Respiration and Oxygen Tension
Daniel S. Shaw, Karlia Meitha, Michael J. Considine, and Christine H. Foyer
12. Isolation of Mitochondria from Model and Crop PlantsSandra M. Kerbler and Nicolas L. Taylor
13. Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in FruitsJurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz Saraiva, Diederson Bortolino Santana, Clesivan Pereira dos Santos, Marcos Goes Oliveira, and José Hélio Costa
14. Measurement of Tricarboxilic Acid Cycle Enzyme Activities in Plants
Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, and Adriano Nunes-Nesi
15. Respiration Traits as Novel Markers for Plant Robustness under the Threat of Climate Change – A Protocol for Validation
Birgit Arnholdt-Schmitt
16. Calorespirometry – A Novel Tool in Functional Hologenomics to Select ‘Green’ Holobionts for Biomass Production
Birgit Arnholdt-Schmitt and Vinod Kumar Patil
17. Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues
Nestor Fernandez Del-Saz, Miquel Ribas-Carbo, Gabriel Martorell, Alisdair R. Fernie, and Igor Florez-Sarasa
18. A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members during Growth and Development
José Hélio Costa and Birgit Arnholdt-Schmitt
19. A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants
José Hélio Costa, Clesivan Pereira dos Santos, Kátia Daniella da Cruz Saraiva, and Birgit Arnholdt-Schmitt
20. Studying Individual Plant AOX Gene Functionality in Early Growth Regulation – A New Approach
Birgit Arnholdt-Schmitt and Vinod Kumar Patil21. Laser-Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L.
Carla Ragonezi and Birgit Arnholdt-Schmitt
22. Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways
Bobba Sunil and Agepati S. Raghavendra23. Measuring Spatial and Temporal Oxygen Flux near Plant Tissues using a Self-Referencing Optrode
Eric S. McLamore, D.M. Porterfield, and Yinglang Wan
