Jain / Raghav / Chavda | Next Generation Green Solvents | Buch | 978-1-041-28870-1 | www.sack.de

Buch, Englisch, 392 Seiten, Format (B × H): 178 mm x 254 mm

Jain / Raghav / Chavda

Next Generation Green Solvents

Emerging Roles of Deep Eutectic Systems
1. Auflage 2026
ISBN: 978-1-041-28870-1
Verlag: Taylor & Francis Ltd

Emerging Roles of Deep Eutectic Systems

Buch, Englisch, 392 Seiten, Format (B × H): 178 mm x 254 mm

ISBN: 978-1-041-28870-1
Verlag: Taylor & Francis Ltd


Deep eutectic solvents (DESs) and natural deep eutectic solvents (NADES) have emerged as transformative materials in the pursuit of sustainable and environmentally responsible chemistry. Their remarkable physicochemical properties, low toxicity, tunable structures, and broad applicability position them as powerful alternatives to conventional solvents and catalysts across diverse scientific and industrial fields. From catalysis, extraction, and electrochemistry to pharmaceuticals, polymerization, agriculture, and energy storage, DESs are redefining modern approaches to green chemical processes and materials design.

This book provides a comprehensive overview of the latest advances in DES and NADES research, covering both fundamental principles and practical applications. It explores the environmental profile, synthesis strategies, physicochemical behavior, and industrial relevance of these innovative solvent systems, while also highlighting emerging developments in machine learning-guided solvent discovery, CO2 recycling, biocatalysis, cryoprotection, and advanced DES@MOF hybrid materials.

Special emphasis is placed on sustainable technologies and industrial translation, demonstrating how DESs can bridge the gap between laboratory innovation and real-world implementation. The book further examines techno-economic considerations, patent landscapes, and challenges associated with large-scale adoption, offering valuable perspectives for future development.

Features:

• Comprehensive coverage of DESs and NADES in sustainable chemistry.

• Emerging applications in catalysis, pharmaceuticals, polymers, agriculture, and energy systems.

• Insights into machine learning, industrial production, and techno-economic aspects.

• Discussion of environmental impact, toxicity, biodegradability, and green processing.

This book serves as a valuable reference for researchers, academicians, industry professionals, and policymakers working in green chemistry, materials science, chemical engineering, and sustainable technologies.

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Chapter 1. Thermodynamic Insights into Deep Eutectic Solvents

1.1. Introduction

1.2. Classification of DESs

1.3. Preparation Method

1.4. Thermal Aspects and Properties of DESs

1.4.1. Phase Transition Characteristics

1.4.2. Melting Point Decrease in Deep Eutectic Solvents

1.4.3. Density

1.4.4. Viscosity

1.4.5. Conductivity

1.4.6. Surface Tension

1.4.7. Optical Density and Refractive Traits of DESs

1.4.8. Polarity

1.4.9. pH

1.5. Hydrophilic and Hydrophobic Behaviour of Deep Eutectic Solvents

1.6. Toxicity of DESs

1.7. Biodegradability

1.8. Effect of Water

1.9. Conclusion

Chapter 2. Unravelling the Environmental Profile of Deep Eutectic Solvents

2.1. Introduction

2.2. DES detection methods 2.2.1. Cytotoxicity assay 2.2.1.1. WST-1 assay 2.2.1.2. MTT/ MTS assay 2.2.2. Phytotoxicity assay 2.2.3. Microbial toxicity bioassay 2.2.3.1. Microtox bioassay 2.2.3.2. Inhibition effect of DES 2.2.4. Biodegradability test

2.3. Environmental Profile 2.3.1. Ecotoxicity 2.3.2. Biodegradability 2.3.3. Renewability 2.3.4. Life Cycle Assessment (LCA)

2.4. Conclusion and outlook

Chapter 3. Novel Green Catalytic Approach for Chemical Transformation Using Deep Eutectic Solvents

3.1. Introduction

3.2. Fundamentals of Deep Eutectic Solvents

3.2.1 Composition and Preparation

3.2.2 Classification

3.2.3 Physicochemical Properties

3.2.4 Comparison with Ionic Liquids

3.3. Synthesis of DESs

3.3.1 Classical Heating and Stirring Method

3.3.2 Solvent-Assisted Method

3.3.3 Mechanochemical and Microwave-Assisted Methods

3.3.4 Aqueous Dilution and Evaporation Method

3.3.5 Mechanistic Insights and Applications

3.4 DESs in Chemical Transformations

3.5 Advantages and Limitations in Catalytic Use

3.6 Future Directions

3.7 Conclusion

3.8 References

Chapter 4. Harnessing the Power of Deep Eutectic Solvents - Applications and Advances

4.1. Introduction

4.2. Need to Harness the Power of Deep Eutectic Solvents (DESs)

4.3. Selected Applications of Deep Eutectic Solvents (DESs) 4.3.1. Extraction and Separation processes 4.3.1.1. Extraction of various environmental pollutants 4.3.1.2. Extraction of various biomolecules 4.3.1.3. Extraction of various metals 4.3.2. Catalysis 4.3.2.1. Homogeneous catalysis 4.3.2.2. Heterogeneous catalysis 4.3.2.3. Biocatalysis 4.3.2.4. Photocatalysis 4.3.2.5. Electrocatalysis 4.3.3. Organic Synthesis 4.3.4. Pharmaceutical and Drug Delivery 4.3.5. Food Industry 4.3.6. Gas Capture 4.3.7. Electrochemistry

4.4. Advances in DESs

4.5. Conclusion

Chapter 5. Deep Eutectic Solvents in Batteries for Sustainable Energy

5.1. Introduction

5.1.1 Structure and chemical composition of DES

5.1.2. Fundamentals of DES

5.2.1 Electrochemical window and electrochemical stability

5.2.2. Ionic conductivity and viscosity

5.2.3 Low Volatility and Nonflammability

5.2.4 Density

5.2. DESs used for energy storage applications

5.2.1 Li-Ion Batteries

5.2.2 Zn-ion Batteries

5.2.3 Al-Ion Batteries

5.2.4 Redox flow batteries

5.2.5 Capacitors

5.3. Recycling of batteries using DESs

5.4. Future perspectives

5.5. Conclusion

Chapter 6. Recent Patents in Deep Eutectic Solvents

6.1. Introduction

6.2. Recent Patents in DES

6.2.1. General DES composition innovations

6.2.2. DES in extraction and resource recovery

6.2.3. DES in food, pharmaceuticals and personal care

6.2.4. DES in Biomass Processing and Recycling

6.2.5. DES in functional materials and environmental applications

6.3. Conclusion and future prospects

Chapter 7. Computational Modeling And Molecular Simulations Of Deep Eutectic Solvents

7.1. Introduction

7.1.1. Defining green chemistry solvents

7.1.2. Fundamental principles and structural features of deep eutectic solvents

7.1.3. The computational imperative: exploring the DES chemical space

7.2. Theoretical Foundations of DESs 7.2.1. Thermodynamics of Eutectic Systems 7.2.2. Integration of knowledge in designing deep eutectic solvents

7.3. Core computational methodologies for DES modelling 7.3.1. Quantum mechanical (QM) approaches 7.3.2. Classical molecular dynamics simulations 7.3.3. Enhanced sampling techniques and free energy

7.4. Molecular docking and solute-DES interactions 7.4.1. General principles of molecular docking

7.4.2. Complexity of docking in functionalized deep eutectic solvents

7.4.3. Docking analysis for predicting solubility for drug compounds and natural extracts 7.4.4. Computational docking and biocatalysis in deep eutectic solvents 7.4.5. Docking, microenvironment and catalytic residues

7.5. Case studies: applications of computation DES research

7.5.1. Biocatalysis and stability of enzymes

7.5.2 Improved bioactive compound extraction

7.5.3. Electrochemical and energy applications (ion transport)

7.5.4. CO2 capture and gas solubility

7.6. Challenges and future directions 7.6.1. The accuracy problem in current force fields 7.6.2. Computational cost for viscous DESs 7.6.3. Machine learning and high-throughput predictive design

7.6.4. Anticipated advances in quantum modelling

7.7. Conclusion

Chapter 8. Superior Polymerization Techniques Using Deep Eutectic Solvents

8.1 Introduction

8. 1.1. Overview of Polymerization Techniques

8.1.2. Evolution of Green Solvent Systems

8.1.3. Scope and Significance of Deep Eutectic Solvents in Polymer Chemistry

8.2. Fundamentals of Deep Eutectic Solvents

8.2.1. Definition and Classification of DESs

8.2.3. DESs vs ILs

8.3. Mechanistic Insights into Polymerization in DESs 8.3.1. Solvent–Monomer Interactions

8.4. Polymerization Techniques Using DESs

8.4.1. Free Radical Polymerization

8.4.2. Controlled/Living Polymerization (RAFT, ATRP, NMP)

8.4.3. Polycondensation and Anionic Polymerizations

8.4.4. Ring opening polymerization using DES

8.4.5. Electrochemical and Photopolymerization Techniques

8.4.6. Enzymatic and Bio-Inspired Polymerizations in DESs

8.4.7. Synthesis of Polymeric Hydrogels using DESs

8.5. Applications and Industrial Relevance

8.5.1. Biomedical Devices and Drug Delivery Systems

8.5.2. Sensors and Flexible Electronics

8.5.3. Water Purification and Environmental Remediation

8.5.4. Coatings, Adhesives, and Packaging

8.5.5 Polymer-Based Electrodes and Membranes for Energy Storage

8.6. Challenges and Future Perspectives

8.6.1. Technical Barriers and Scalability Issues

8.6.2. Regulatory and Safety Considerations

8.6.3. Integration with Circular Economy and Bioeconomy

8.6.4 Emerging Trends in DES Formulation and Polymer Design

8.7.Conclusion

Chapter 9. Beyond Traditional Uses: Enhancing Drug Solubility and Compatibility with Deep Eutectic Solvents

9.1. Introduction

9.2. Drug Solubility Enhancement: The Challenges and Strategic Solutions

9.3. Insights into the Thermodynamics and Microstructure of DES

9.4. Characterization of DESs 9.4.1. Thermal Analysis 9.4.1.1. Differential Scanning Calorimetry 9.4.1.2. Thermogravimetric Analysis (TGA) 9.4.2. X-Ray Diffraction Analysis 9.4.3. Vibrational Analysis 9.4.3.1. Fourier Transform Infrared Spectroscopy (FTIR) 9.4.3.2. Raman Spectroscopy 9.4.4. Microscopic Analysis 9.4.4.1. Polarized Optical Microscopy (POM)  9.4.4.2. Hot Stage Microscopy (HSM)  9.4.4.3. Scanning Electron Microscopy (SEM) 9.4.5. Nuclear Magnetic Resonance (NMR) Spectroscopic Analysis 9.4.5.1. Solid State NMR 9.4.5.2. Pulsed field gradient NMR (PFG-NMR)

9.4.5.3. 1H and 13C NMR 9.4.6. Theoretical assessment of DES

9.5. Classification of DESs 9.5.1. Natural Deep Eutectic Solvents (NADESs) 9.5.2. Therapeutic Deep Eutectic Solvents (THEDES) 9.5.2.1. THEDES as Carrier For API 9.5.2.2. One Component API Based THEDES 9.5.2.2.1. Terpene-based DES 9.5.2.2.2. Fatty acid-based DES 9.5.2.2.3. Organic acid-based THEDES 9.5.2.2.4. Amino acid-based DES 9.5.2.3. Two Component API Based THEDES 9.5.2.4. THEDES Free from APIs 9.5.3. Ternary DES

9.6. Role of THEDES in Maximising Drug Delivery for Disease Treatment 9.6.1. Role of THEDES in Promoting Antimicrobial Activity of Drugs 9.6.2. Anti-inflammatory and Wound Healing Properties of THEDES 9.6.3. Role of THEDES in Anti-tuberculosis Therapy 9.6.4. Role of THEDES in Diabetes Treatment 9.6.5. Role of THEDES in Anticancer Therapies

9.7. Pharmacodynamic and Pharmacokinetic Studies of API-based DES

9.8. Solid Pharmaceutical Dosage formation with EMs

9.9. Limitations and Challenges Associated with the Pharmaceutical Applications of DES

9.10. Concluding remarks

Chapter 10. Metal-organic framework accomplished by Deep Eutectic Solvents

10.1. Introduction

10.2. DES and MOFs

10.2.1. Fundamentals of DES

10.2.2. MOFs and MOFs as Adsorbents

10.3. Impact of DES on MOFs

10.3.1. Effect of DES on MOF Architecture

10.3.2. DES Effects on MOF Performance

10.4. MOF synthesis in DES

10.4.1. MOF Synthesis in DESs of the Choline Chloride/Urea Type

10.4.1.1. Phosphonate-Based MOFs

10.4.1.2. Zeolitic Imidazolate Frameworks (ZIFs)

10.4.1.3. Carboxylate-Based MOFs

10.4.2. MOFs synthesised in non-urea DESs

10.5. DES-integrated MOFs for sample preparation

10.5.1. Methodologies for incorporating DES into MOFs

10.5.2. Extraction Technologies Coupled with DES@MOF Composites

10.5.2.1. Solid-phase extraction (SPE)

10.5.2.2. Solid-phase microextraction (SPME)

10.5.2.3. Dispersive SPE (DSPE) and magnetic SPE (MSPE)

10.5.2.4. Molecularly imprinted solid-phase (micro) extraction (MISPE)

10.6. Post-synthetic modification of MOFs using DES

10.6.1. MOFs dispersed in DES

10.6.2. DES@MOF composites

10.7. Applications of DES@MOFs

10.7.1. Sorptive extraction

10.7.1.1. Pharmaceutical analysis

10.7.1.2. Dye analysis

10.7.1.3. Analysis of heavy metals

10.7.1.4. Pesticide analysis

10.7.2. Adsorption and degradation of emerging organic contaminants (EOCs)

10.7.3. Electrochemical and optical sensing applications

10.7.4. Catalytic applications in organic transformations

10.7.5. Energy storage and gas separation technologies

10.7.6. Wastewater treatment and environmental remediation

10.8. Conclusions and future prospects

Chapter 11. NADES as Catalysts and Sustainable Reaction Media: Green Chemistry Perspectives

11.1. Introduction

11.2. Fundamentals of NADES: From Composition to Catalytically Relevant Properties

11.2.1. Components of NADES and its Functional Landscape

11.2.2. Solvation Dynamics and Microheterogeneity in NADES

11.2.3. Catalysis-relevant Physicochemical Properties

11.2.4. Assessment of green metrics and sustainability for NADES in catalysis

11.3. NADES-based Solvent as Reaction Media for Chemical Transformations

11.3.1. NADES as Environment Friendly Safer Replacements

11.3.2. Substrate solubility, microstructure and reactivity in NADES

11.3.3. ILs (Ionic Liquids) and DESs vs NADES: Strengths and Weaknesses

11.3.4. Operating conditions and intensification in NADES-based processes

11.3.5. Representative reactions in NADES media

11.4. Beyond solvents, when the medium becomes an organocatalyst /co-catalyst

11.4.1. Acidic NADES as dual solvent-catalyst systems

11.4.2. Transition state stabilisation via NADES’s hydrogen bond network

11.4.3. Basic NADES for Base-Catalyzed Processes

11.4.4. Chiral NADES as Stereoselective Organocatalysts

11.5. Homogeneous and heterogeneous metal catalysis in NADES media

11.5.1. Homogeneous metal catalysis

11.5.2. Heterogeneous metal catalysis and metal nanoparticle stabilisation by NADES Media

11.5.3. Ligand Free Metal Catalysis via NADES

11.5.4. Recovery and Recycling of Catalyst

11.6. Future Prospects, Challenges and Limitations

11.7. Conclusion

Chapter 12. Natural Deep Eutectic Solvents in Advanced Drug Delivery Systems

12.1. Introduction

12.2. Classification and Fundamental Properties of NADES 12.2.1 Sugar-based NADES in Drug Delivery Systems 12.2.2 Organic acid-based NADES in Drug Delivery Systems 12.2.3 Amino acid-based NADES in Drug Delivery Systems 12.2.4 Terpenoid-based NADES in Drug Delivery Systems

12.3. NADES-based Drug Delivery Routes 12.3.1 Oral Routes for NADES-based Drug Delivery 12.3.2 Transdermal and Topical Routes for NADES-based Drug Delivery 12.3.3 Mucosal Routes for NADES-based Drug Delivery

12.4. Challenges and Future Research Directions

12.5. Conclusion

Chapter 13. Bio-Inspired Antifreezing Strategies in Foods Using Natural Deep Eutectic Solvents

13.1.Introduction

13.2. Fundamentals of Natural Deep Eutectic Solvents

13.2.1. Definition and Classification

13.2.2. Formulation Techniques and Molecular Compositions

13.2.3. Physicochemical Properties of Cryoprotection interest

13.3. Mechanisms of Bio-inspired NADES-Mediated Cryoprotection

13.3.1. Hydrogen Bonding Networks and Water Immobilization

13.3.2. Ice Nucleation Suppression

13.3.3. Ice Recrystallization Inhibition

13.3.4. Glass Transition Behaviour and Vitrification

13.4. Water-Tailoring and its effects on Antifreezing

13.5. Applications in Food Systems

13.5.1. Microbial Cryopreservation

13.5.2. Muscle Foods and Surimi Products

13.5.3. Emulsion Systems and Pickering Emulsions

13.6. Structure-Function Relationships

13.7. Challenges and Limitations

13.7.1. Stability Issues and Amadori Rearrangement

13.7.2. Viscosity and Mass Transfer Limitations

13.7.3. Scale-Up and Industrial Implementations

13.7.4. Regulatory and Safety Considerations

13.8. Conclusion

Chapter 14. Natural Deep Eutectic Solvent vs. Ionic Liquids: Comparative Insights into Structure, Properties, and Applications

14.1. Introduction

14.2. NADES: Formation, Structure, and Roles 14.2.1. Synthesis of NADES 14.2.2 Roles of NADESs in Organisms 14.2.3 Structure of NADES

14.3 Physicochemical Properties of NADESs

14.4 Synthesis of ILs

14.5 Physicochemical Properties of ILs

14.5.1 Density

14.5.2 Viscosity

14.5.3 Surface tension

14.5.4 Thermal conductivity

14.6 Task Specific Ionic Liquids (TSILs)

14.7 An assessment of the main synthesis processes of ILs and DESs

14.8 Applications of NADESs

14.8.1 Using NADESs for Biocatalysis

14.8.2 Using NADESs for Extraction

14.8.3 NADESs for Biomass Pretreatment

14.8.4 NADESs for Clinical Treatment

14.8.5 NADESs for Pharmaceutical and Nutraceutical Product Preparation

14.8.6 NADESs for Electrochemical Detection of Bioactive Materials

14.9 Applications of ILs

14.9.1 Use of ILs as Catalysts

14.9.2 ILs as Soluble Supports

14.9.3 ILs in electrolyte applications

14.9.4 ILs in biomedical applications

14.10 Advantages and Drawbacks

14.11 Future Perspectives and Conclusion

Chapter 15. NADES in Agriculture: Toward Sustainable and Environmentally Friendly Farming Practices

15.1. Introduction

15.2. Composition, Formation, and Types of NADES

15.3. Physicochemical Properties and Advantages Over Conventional Solvents

15.4. Role of NADES in Enhancing Nutrient Uptake and Plant Growth

15.5. NADES-Based Bioformulations for Biopesticides and Biocontrol Agents

15.6. Application of NADES in Seed Priming and Germination Efficiency

15.7. NADES as Carriers for Phytoactive Compounds and Bioinoculants

15.8. Effect of NADES on the Soil Health Improvement and Enhancement of Microbial Activity

15.9. Mitigation of Abiotic Stress through NADES-Mediated Signaling

15.10. Integration of NADES in Organic and Precision Agriculture Systems

15.11. Environmental Impact, Biodegradability, and Safety Assessment

15.12. Commercial Prospects, Limitations, and Technological Challenges

15.13. Future Directions for Scalable NADES-Based Agricultural Practices

15.14. Conclusion

15.15. References

Chapter 16. Combating Restrictions and Control Limits in Deep Eutectic Solvents

16.1 Introduction

16.2 Combating Restrictions and Control Limits 16.2.1 High Viscosity

16.2.2 Thermal and Chemical Instability

16.2.3 Limited Recyclability and Regeneration (i) Nature of the Problem (ii) Challenges in Regeneration (iii) Industrial Implications

16.2.4 Incomplete Toxicological Profiling

16.2.5 Regulatory Hurdles in Commercializing DESs

16.2.6 Limited Solubility Scope

16.2.7 Enzyme Compatibility and Biocatalysis Limitations

16.2.8 Phase Behavior and Water Sensitivity

16.2.9 Limited Component Diversity and Optimization

16.2.10 Scalability and Industrial Integration Challenges

16.2.11 Inadequate Data on Environmental Fate

16.2.12 Side Reactions and Chemical Incompatibility

16.3 Conclusion

Chapter 17. Commercialization of Deep Eutectic Solvents: Trends and Prospects

17.1. Introduction

17.2. Fundamental properties of DESs

17.3. Application areas of DESs with commercial potential

17.4. Current status of DESs commercialization

17.5. DESs Market and Global Perspective on DESs Progress

17.6. Techno-economic feasibility analysis of DESs

17.7. Challenges hindering commercialization

17.8. Strategies for commercial advancement

17.9. Conclusion


Prof. Pallavi Jain

Prof. Jain has a very luminous academic and professional career. She is currently heading the Student Welfare Department at SRM Institute of Science and Technology, Delhi-NCR Campus, Modinagar, Ghaziabad, India. Dr. Jain received her Ph.D. degree in Chemistry from the Department of Chemistry, Banasthali Vidyapith, Rajasthan, India, in 2017. She has extensive teaching experience of about 20 years. Her research interests focus on the development of transition metal complexes incorporating Schiff base ligands for their biological importance in drug delivery. Her research interests also focus on the synthesis of Deep eutectic solvents. She has authored and co-authored over 85 publications in journals of international repute and contributed more than 65 book chapters. The citation of her work is more than 1550. She has also edited five books.  Dr. Sapna Raghav

Dr. Sapna Raghav obtained her Ph.D. in Chemistry from Banasthali University, Rajasthan, in 2019. Her research interests are centered on Environmental Chemistry, with particular emphasis on water decontamination, chemical sensing, and advanced materials. Dr. Raghav has made significant scholarly contributions, with over 35 research articles published in reputed international journals, including Carbohydrate Polymers, ACS Publications, and the Journal of Molecular Liquids. She has also authored more than 40 book chapters for leading academic publishers such as Elsevier, Springer, Wiley, and CRC Press. In addition, she has edited three books published by Springer and one volume by CRC Press. With over five years of teaching experience, Dr. Raghav currently serves as the Head of the Department of Chemistry at Shree Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan. Her academic impact is reflected in her strong research metrics, including an h-index of 17, an i10-index of 27, and more than 1,100 citations, highlighting her contributions to environmental and materials chemistry.

Dr. Vishwajit Chavda

Dr. Vishwajit Chavda, Ph.D., is a Postdoctoral Researcher in the Department of Environmental Engineering at Kyungpook National University, Daegu, South Korea, specializing in environmental and materials chemistry. He received his Ph.D. in Applied Chemistry from The Maharaja Sayajirao University of Baroda, India, in 2024 under the supervision of Prof. Sanjeev Kumar. He completed his B.Sc. and M.Sc. in Chemistry from Gujarat University in 2020 and earned a PG Diploma in Intellectual Property Rights from Gujarat National Law University in 2023. He has authored over 28 research articles in Scopus-indexed journals, edited 3 book volumes, 13 book chapters, and 7 Indian patents, with ~450 citations and an h-index of 14. His research contributions have been featured in Indian media and highlighted on the cover pages of ACS and Wiley journals. He actively serves as a reviewer for journals published by Elsevier, ACS, RSC, Wiley, Springer, Bentham, and Taylor & Francis (reviewed 200+ articles), and holds lifetime memberships in several scientific societies. His research focuses on nanomaterials, wastewater treatment, environmental remediation, and green/sustainable chemistry, with particular emphasis on advanced functional materials for sustainable applications.

Prof. Woong Kim

Prof. Woong Kim is a professor of Environmental Engineering at Kyungpook National University, South Korea. He obtained his Ph.D. degree from a prestigious Korean institute (POSTECH). He published more than 100+ papers in reputed SCOPUS and SCIE journals, with ~7200 citations and an h-index of 48. He is currently associate editor of ‘Biodegradation’, a Springer Nature journal. Also, an editorial board member of 'Microbiology and Biotechnology Letter'. His lab focuses research on various streams like Anaerobic Digestion, Wastewater Treatment, Microalgal Cultivation for Biodiesel Production, Life Cycle Assessment, Electrochemical Harvest of Microalgae, Exploratory Data Analysis Process Performance and Microbial Community, Process Optimization Using Response Surface Methodology, Statistical Analysis of Microbial Community Structure and Dynamics.



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