Understanding PCR

A Practical Bench-Top Guide
2. Auflage 2027
ISBN: 978-0-443-52526-1
Verlag: Elsevier Science

Buch, Englisch, 150 Seiten, Format (B × H): 152 mm x 229 mm

“Understanding PCR: A Practical Bench-Top Guide", second edition, delivers a comprehensive resource offering researchers the information they need to plan their polymerase chain reactions, from reagents and conditions to analysis and beyond. This updated edition covers a broad spectrum of topics including laboratory preparation, primer design, DNA analysis, quantitative PCR, cloning, protein expression, and the latest applications. The book equips researchers with the knowledge to confidently design and execute PCR experiments, enhancing reproducibility and efficiency. By integrating updated techniques and practical insights, this revised reference supports researchers, laboratory professionals, and students working across molecular biology and related fields.

Autoren/Hrsg.

Jenkins, Rowena

Parker, Josie

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Things to consider before preparing to undertake your first polymerase chain reaction
2. Designing and ordering your polymerase chain reaction primers
3. Analyzing your template DNA and/or PCR product
4. Quantitative PCR: things to consider
5. Carrying Out Q-PCR
6. Using PCR for cloning and protein expression
7. Using PCR for CRISPR-Cas9 genome editing

Über Autor(innen)

Jenkins, Rowena
Rowena Jenkins, PhD, is currently a medical writer at Health Economics and Outcomes Research Ltd, UK. Prior to this she served as lecturer in Microbiology and Infectious Disease in the College of Medicine at Swansea University, UK. Her research team are focused on the effect of novel antimicrobial agents on pathogenic bacteria found in clinical infections, particularly chronic infections such as those seen in diabetic foot ulcers and cystic fibrosis lungs infection, and the impact on the microbiome within those conditions. Dr. Jenkins also has an interest in the potential for natural antimicrobial agents to improve the efficacy of conventional antibiotics against antibiotic resistant bacteria and biofilms. Dr. Jenkins serves as a section editor for the Journal of Medical Microbiology, and has co-authored two books with Elsevier, including the first edition of "Understanding PCR".

Parker, Josie
Josie Parker, PhD, is a lecturer in Biochemistry at Cardiff University, UK. Her research focuses on cytochrome P450 enzymes which are the targets of inhibitors such as antifungal and chemotherapy agents and have capabilities that can be harvested for biotechnology. She studies the physiological function of these enzymes in various microorganisms and their targeting as potential drug targets. Her research has led to discoveries in antifungal resistance and the treatment of fungal infections in both medicine and agriculture. Dr. Parker has contributed to almost 70 publications, is a Fellow of the Higher Education Academy and an STEM Ambassador.