Buch, Englisch, Band 1429, 198 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 5489 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1429, 198 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 5489 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-3620-5
Verlag: Springer
This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in Plant Cytogenetics: Methods and Protocols build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting
edge and thorough, Plant Cytogenetics: Methods and Protocols, is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-Color FISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous Chromosomes.- Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabeling and In Situ Labelling of Isolated Plant Interphase Nuclei.- Manipulation of Homologous Chromosome Recombination in Wheat.- Dissecting Plant Chromosomes by the Use of Ionizing Radiation.- Optical Nano-Mapping and Analysis of Plant Genomes.- Flow Sorting Plant Chromosomes.- Construction of BAC Libraries from Flow-Sorted Chromosomes.- The Chromosome Microdissection and Microcloning Technique.- Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis.- Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation.- Chromatin Immunoprecipitation to Study Plant Epigenome.
