Buch, Englisch, Band 3074, 544 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1174 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 3074, 544 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1174 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5538-2
Verlag: Springer
This third edition provides new and updated experimental and computational methods designed to study transcriptional regulation in stem cells and related contexts. Chapters highlight genomic profiling, single cell and multiomic technologies, chromatin and spatial analysis, and computational modeling. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, aims to provide practical guidance for investigators seeking to implement these approaches in their own research.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Inferring Gene Regulatory Networks in Stem Cells: Methods and Applications.- Computational network analysis for defining transcriptional programs.- Deep Learning Protocols for Predicting Drug Mechanism of Action and Drug–Target Interactions.- Topic-Driven Bibliometrics and Trend Intelligence for Stem Cell and Cancer Research.- TORC: Target-Oriented Reference Construction for supervised cell-type identification in scRNA-seq.- A standardized protocol for generating iPSC-derived human microglia for functional genomic assays.- A Defined Strategy for Multi-lineage Differentiation of Gastric Organoids.- Generation of Transgene-Free Naive Human Induced Pluripotent Stem Cells from Somatic Cells Using a Modified Temperature-Sensitive Sendai Virus System.- Skeletal muscle tissue engineering: methods and analysis for the bio-fabrication and validation of a 3D scaffold-free muscle construct.- Preparation of Mesenchymal Stromal Cells-derived Extracellular Vesicles as carriers for Paclitaxel delivery.- Systematic Analysis of Tumor Microenvironment Using IOBR.- Systematic Dissection of Key Driver Perturbation Signatures in Single Cells via ECCITE-seq.- B cell aware analysis of single-cell transcriptomics data.- Combined Lipidomic and Metabolomic Analyses on Cardiac Organoids.- A cell atlas of adult Aedes aegypti midgut revealed by single nucleus/cell RNA sequencing.- Genomic Profiling of Chromatin State Using CUT&Tag.- Identification of Genome-Wide Chromatin Structural Aberration in Cancer by Hi-C analysis.- Mapping stage-specific enhancer dynamics during the specification of human trophoblast lineage.- Preparation of postmortem human formalin fixed paraffin embedded frontal cortex tissue for profiling pyramidal neurons via digital spatial profiling.- Improving Image Quality in 10x Visium Spatial Transcriptomics Using Vispro.- Advanced Image Generation for Cancer and Stem Cell Biology Using Diffusion Models.- Automated Quantification and Morphometric Analysis of Pluripotent Stem Cell Colonies Using ColonyQuant.- Alkaline Phosphatase Staining of Embryonic Stem Cells.- Lentiviral Transduction of Embryonic Stem Cells.- Teratoma Formation and Genomic Profiling Using Multi-omics Approaches.- Wound Healing Assay.- Characterization of the Senescence-Associated Secretory Phenotype in Primary and Induced Pluripotent Stem Cell-Derived Skin Cells via Multiplex Assays.- Decoding Nano-Bio Interactions: Gene Regulatory Networks in Advanced Drug Loading.- Integrative Transcriptomic Network Modeling Coupled with Patient-Derived Validation for Circulating lncRNA Biomarker Discovery in NAFLD: A Comprehensive Workflow.- Enhancing the functionality of human bone marrow-derived mesenchymal stromal cells using signaling modulators.
