Single Molecule Biology

Erscheinungsjahr 2008
ISBN: 978-0-12-374227-8
Verlag: Elsevier Science

Buch, Englisch, 368 Seiten, Format (B × H): 198 mm x 244 mm, Gewicht: 920 g

Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery.

Zielgruppe

Single molecule biology is an inherently cross-disciplinary field of research, so this book appeals to workers from a broad range of disciplines, including structural biologists, biophysicists, cell biologists, and biochemists. The target audience is advanced undergraduates, graduates, postgraduates, and researchers.

Autoren/Hrsg.

Knight, Alexander E

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Introduction and Historical Perspective

Motor Proteins: Linear Motors

Actin and Tubulin-Based Motors

DNA Motors
Rotary Motors

Single Molecule Enzymology

Ribozymes

Ribosomes

Large Complexes

Membrane Proteins

Channels and Pores
Cytoskeleton

DNA Mechanics and Structure

Single Molecules in Cells

Hair Cells

Structure of Single Protein Molecules

Single Molecule Mechanical Properties

Single Molecule Applications: Diagnostics

Single Molecule Applications: Arrays
Single Molecule Applications: Drug Discovery