Advanced Techniques in Biological Electron Microscopy

Softcover Nachdruck of the original 1. Auflage 1973
ISBN: 978-3-642-65494-7
Verlag: Springer

Buch, Englisch, 306 Seiten, Paperback, Format (B × H): 152 mm x 229 mm, Gewicht: 466 g

The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu­ tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec­ tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad­ vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful­ ness in the near future.

Zielgruppe

Research

Autoren/Hrsg.

Koehler, J. K.

Weitere Mitwirkende

Bullivant, S.

Frank, J.

Hama, K.

Hayes, T.L.

Luft, J.H.

McHenry, F.A.

Pease, D.C.

Salpeter, M.M.

Inhaltsverzeichnis

Embedding Media — Old and New.- A. Introduction.- B. Early Embedding Media.- C. Conventional Embedding Media.- D. Advantages and Disadvantages of Conventional Embedding Media.- E. New Embedding Media.- F. Conclusion.- References.- Substitution Techniques.- A. Introduction.- B. Inert-dehydration.- C. Freeze-substitution.- D. Conclusions.- References.- Freeze-Etching and Freeze-Fracturing.- A. Introduction.- B. Freezing of Biological Systems.- C. Methods and Instrumentation.- D. Interpretation.- E. Conclusions.- References.- Electron Microscope Autoradiography.- F. A. McHenry.- A. Introduction.- B. Distribution of Developed Grains Around Radioactive Sources.- C. Analysis of Autoradiograms.- D. Conversion of Developed Grain Data to Information on Radioactivity.- References.- Scanning Electron Microscope Techniques in Biology.- A. Introduction.- B. Specimen Preparation.- C. Viewing Techniques.- D. Signal Processing.- E. Recording Techniques.- F. Information Assimilation by the Observer.- 3. Electrical Properties and Charging.- G. Conclusion.- H. Appendices.- I. Optical Aids for the Viewing of Vertically Mounted Stereo-Pairs.- References.- Computer Processing of Electron Micrographs.- A. Introduction.- B. Linear Systems and Fourier Processing.- C. Digitizing of Electron Micrographs.- D. Noise Filtering.- E. The Cross Correlation Function and its Use for Image Alignment.- F. Two-Dimensional Restoration.- G. Object/Support Separation.- H. Three-Dimensional Reconstruction.- References.- High Voltage Electron Microscppy.- A. Introduction.- B. Merits of the High Voltage Electron Microscope.- C. Biological Applications.- D. Design and Construction of High Voltage Electron Microscopes.- References.