Buch, Englisch, 633 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 633 Seiten, Format (B × H): 178 mm x 254 mm
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-5662-4
Verlag: Springer Us
This detailed volume provides a comprehensive yet accessible account of various molecular marker systems and their practical applications. Accounting for early methods like Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD), the book explores Single Nucleotide Polymorphism (SNP) markers and high-throughput sequencing technologies that have further revolutionized the field, enabling large-scale genomic analysis with remarkable accuracy. Each method is described with careful attention to essential steps such as DNA extraction, quantification, amplification, and analysis. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, serves as an ideal resource to inspire further exploration and innovation in the use of molecular markers, leading to the effective application of these techniques to sustainable agriculture and biodiversity conservation.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Naturwissenschaften Biowissenschaften Botanik
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
Weitere Infos & Material
Application of Sequence-Related Amplified Polymorphism (SRAP) Markers for Assessing Molecular Diversity of Trichoderma Associated with Biocontrol Potential Against Phytopathogen.- Utilizing RNA-Seq-Derived SNPs for KASP Marker Development in Maize.- Assessment of Clonal Integrity and Virus Indexing in Micro-Propagated Sugarcane Using DNA-Based Markers.- Application of ISSR Molecular Markers in Field Crops.- RAPD Markers for the Study of Genetic Diversity in Echium amoenum Ecotypes.- In Silico Pipeline for the Development and Primer Design of Microsatellite Markers in Mangifera similis Blume.- Computational and qPCR-Based Determination of Gene Copy Number as a Molecular Marker for Transgenic Plant Analysis.- Application of SSR Markers for the Detection of Metal Tolerance in Plants.- RAPD Marker Optimization and Genetic Diversity Assessment in Averrhoa Genus.- Development and Validation of SNP Markers for Plant Genomic Studies.- Exploring the Role of Single Nucleotide Polymorphisms in Modern Plant Genomic Research.- Development and Application of Random Amplified Polymorphic DNA (RAPD) for the Improvement of Cotton Crops.- Development and Application of Restriction Fragment Length Polymorphism (RFLP) for the Improvement of Cotton Crops.- Development and Application of Expressed Sequence Tags (ESTs) for the Improvement of Cotton Crops.- Development of Single Nucleotide Polymorphism (SNP) Markers in Sesame (Sesamum indicum L.).- Application of Single Nucleotide Polymorphisms (SNPs) in Sesame (Sesamum indicum L.) for Genetic Diversity, Mapping, and Marker-Assisted Selection.- ISSR-PCR for Genetic Diversity Analysis in Faba Bean.- Development of SNPs for Crop Improvement.- Non-Sequencing-Based Microsatellite Development in Pomegranate (Punica granatum): Enrichment and Screening Protocol.- Development of Genome-Wide Simple Sequence Repeat Markers from Whole-Genome Sequence of Mungbean (Vigna radiata).- SNP Discovery of 3K RICE Panel for Specific Traits.- Application of Restriction Fragment Length Polymorphism (RFLP).- Application of Molecular Markers for Trait-Specific Breeding of Aroma Accumulation in Rice.- Development and Application of Single Nucleotide Polymorphism (SNP) Markers for the Identification of Pest and Disease Resistance in Sugarcane.- Development and Applications of Single Nucleotide Polymorphisms (SNPs) for the Improvement of Fiber Traits in Cotton Crops.- SSR Marker-Assisted Selection and Monitoring of High-Metabolite Cell Lines in Plant Tissue Culture: Protocols and Somaclonal Variation Assessment.- ISSR Marker-Based Authentication of In Vitro-Derived Medicinal Plant Lines for Enhanced Phytochemical Production.
