Molecular Motors

Methods and Protocols
2. Auflage 2018
ISBN: 978-1-4939-8554-8
Verlag: Springer

Buch, Englisch, Band 1805, 452 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1063 g

Reihe: Methods in Molecular Biology

This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicists and molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lavelle, Christophe

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Preface…Table of Contents…Contributing Authors…
1. Cellular and Nuclear Forces: An OverviewBidisha Sinha, Arikta Biswas, and Gautam V. Soni
Part I Membrane Motors
2. The Bacterial Flagellar Rotary Motor in ActionNicholas J.S. Delalez and Judith P. Armitage
3. Purification and Reconstitution of Ilyobacter tartaricus ATP SynthaseGanna O. Krasnoselska and Thomas Meier
Part II Cytoskeletal Motors
4. Using Microfluidics Single Filament Assay to Study Formin Control of Actin AssemblyGuillaume Romet-Lemonne, Bérengère Guichard, and Antoine Jégou
5. Engineering Synthetic Myosin Filaments using DNA NanotubesRuth F. Sommese and Sivaraj Sivaramakrishnan
6. Direct Imaging of Walking Myosin V by High-Speed Atomic Force MicroscopyNoriyuki Kodera and Toshiro Ando
7. High-Resolution Single-Molecule Kinesin Assays at kHz Frame RatesKeith J. Mickolajczyk and William O. Hancock
8. Multicolor Tracking of Molecular Motors at Nanometer ResolutionSara Wichner and Ahmet Yildiz
9. High-Speed Optical Tweezers for the Study of Single Molecular MotorsL. Gardini, A. Tempestini, F.S. Pavone, and M. Capitanio
10. Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force MicroscopyMatthew Batchelor, Marcin Wolny, Marta Kurzawa, Lorna Dougan, Peter J. Knight, and Michelle Peckham
Part III Nucleic Acids Motors
11. The Role of Supercoiling in the Motor Activity of RNA PolymerasesAnnick Lesne, Jean-Marc Victor, Edouard Bertrand, Eugenia Basyuk and Maria Barbi
12. Single-Molecule FRET Analysis of Replicative HelicasesSeung-Jae Lee, Salman Syed, and Taekjip Ha
13. Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular MicroscopyPauline Dupaigne, Eliana Moreira Tavares, Olivier Piétrement, and Eric Le Cam
14. DNA Organization and Segregation Super-ResolvedDiego I. Cattoni, Jean-Bernard Fiche, Antoine Le Gall, and Marcelo Nollmann
15. Electrophoretic Analysis of the DNA Supercoiling Activity of DNA GyraseBelén Martínez-García, Antonio Valdés, Joana Segura, Ofelia Díaz-Ingelmo, and Joaquim Roca
16. Single Molecule Angular Optical Trapping for Studying Transcription under TorsionJie Ma, Chuang Tan, and Michelle D. Wang
17. Anisotropy-Based Nucleosome Repositioning AssayKoan Briggs, Gada Al-Ani, Allen Eastlund, and Christopher J. Fischer
18. Remodeling and Repositioning of Nucleosomes in Nucleosomal ArraysJohanna Ludwigsen, Nicola Hepp, Henrike Klinker, Sabrina Pfennig, and Felix Mueller-Planitz
19. Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers Damian J. delToro and Douglas E. Smith
20. Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical TweezersNicholas Keller, Damian J. delToro, and Douglas E. Smith
21. Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging MotorFarzin Haque, Hui Zhang, Shaoying Wang, Chun-Li Chang, Cagri Savran, and Peixuan Guo